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1sxt

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(New page: 200px<br /><applet load="1sxt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sxt, resolution 2.7&Aring;" /> '''STAPHYLOCOCCAL ENTERO...)
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[[Image:1sxt.gif|left|200px]]<br /><applet load="1sxt" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sxt, resolution 2.7&Aring;" />
 
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'''STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC'''<br />
 
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==Overview==
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==STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC==
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Superantigens form complexes with major histocompatibility complex (MHC), class II molecules and T-cell receptors resulting in extremely strong, immunostimulatory properties. Staphylococcus aureus enterotoxin A (SEA), belongs to a subgroup of the staphylococcal superantigens that utilizes, Zn2+ in the high affinity interaction with MHC class II molecules. A high, affinity metal binding site was described previously in SEA, co-crystallized with Cd2+ in which the metal ion was octahedrally, co-ordinated, involving the N-terminal serine. We have now co-crystallized, SEA with its native co-factor Zn2+ and determined its crystal structure at, 2.7 A resolution. As expected for a Zn2+ ion, the co-ordination was found, to be tetrahedral. Three of the ligands are located on the SEA surface on, a C-terminal domain beta-sheet, while the fourth varies with the, conditions. Further analysis of the zinc binding event was performed using, titration microcalorimetry, which showed that SEA binds Zn2+ with an, affinity of KD = 0.3 microM in an entropy driven process. The differential, Zn2+ co-ordination observed here has implications for the mechanism of the, SEA-MHC class II interaction.
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<StructureSection load='1sxt' size='340' side='right'caption='[[1sxt]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1sxt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SXT FirstGlance]. <br>
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1SXT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SXT OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxt OCA], [https://pdbe.org/1sxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sxt RCSB], [https://www.ebi.ac.uk/pdbsum/1sxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxt ProSAT]</span></td></tr>
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The Co-crystal structure of staphylococcal enterotoxin type A with Zn2+ at 2.7 A resolution. Implications for major histocompatibility complex class II binding., Sundstrom M, Hallen D, Svensson A, Schad E, Dohlsten M, Abrahmsen L, J Biol Chem. 1996 Dec 13;271(50):32212-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8943278 8943278]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/ETXA_STAAU ETXA_STAAU] Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome. The illness is characterized by high fever, hypotension, diarrhea, shock, and in some cases death.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sx/1sxt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sxt ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Sundstrom, S.M.]]
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[[Category: Sundstrom SM]]
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[[Category: ZN]]
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[[Category: enterotoxin]]
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[[Category: staphylococcus]]
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[[Category: superantigen]]
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[[Category: toxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:49:42 2007''
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Current revision

STAPHYLOCOCCAL ENTEROTOXIN TYPE A (SEA) CO-CRYSTALLISED WITH ZINC

PDB ID 1sxt

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