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1sz0

From Proteopedia

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(New page: 200px<br /><applet load="1sz0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sz0, resolution 2.10&Aring;" /> '''N-terminal 3 domains...)
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[[Image:1sz0.gif|left|200px]]<br /><applet load="1sz0" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sz0, resolution 2.10&Aring;" />
 
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'''N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate'''<br />
 
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==Overview==
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==N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate==
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The 300-kDa cation-independent mannose 6-phosphate receptor (CI-MPR) plays, a critical role in the trafficking of newly synthesized mannose, 6-phosphate-containing acid hydrolases to the lysosome. The receptor, contains two high affinity carbohydrate recognition sites within its, 15-domain extracytoplasmic region, with essential residues for, carbohydrate recognition located in domain 3 and domain 9. Previous, studies have shown that these two sites are distinct with respect to, carbohydrate specificity. In addition, expression of truncated forms of, the CI-MPR demonstrated that domain 9 can be expressed as an isolated, domain, retaining high affinity (Kd approximately 1 nm) carbohydrate, binding, whereas expression of domain 3 alone resulted in a protein, capable of only low affinity binding (Kd approximately 1 microm) toward a, lysosomal enzyme. In the current report the crystal structure of the, N-terminal 432 residues of the CI-MPR, encompassing domains 1-3, was, solved in the presence of bound mannose 6-phosphate. The structure reveals, the unique architecture of this carbohydrate binding pocket and provides, insight into the ability of this site to recognize a variety of, mannose-containing sugars.
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<StructureSection load='1sz0' size='340' side='right'caption='[[1sz0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sz0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZ0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M6P:ALPHA-D-MANNOSE-6-PHOSPHATE'>M6P</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=OS:OSMIUM+ION'>OS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sz0 OCA], [https://pdbe.org/1sz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sz0 RCSB], [https://www.ebi.ac.uk/pdbsum/1sz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sz0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MPRI_BOVIN MPRI_BOVIN] Acts as a positive regulator of T-cell coactivation, by binding DPP4 (By similarity). Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1sz0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sz0 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with NAG, OS and M6P as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SZ0 OCA].
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*[[Insulin-like growth factor receptor|Insulin-like growth factor receptor]]
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__TOC__
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==Reference==
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</StructureSection>
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The N-terminal carbohydrate recognition site of the cation-independent mannose 6-phosphate receptor., Olson LJ, Dahms NM, Kim JJ, J Biol Chem. 2004 Aug 6;279(32):34000-9. Epub 2004 May 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15169779 15169779]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dahms, N.M.]]
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[[Category: Dahms NM]]
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[[Category: Kim, J.J.P.]]
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[[Category: Kim J-JP]]
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[[Category: Olson, L.J.]]
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[[Category: Olson LJ]]
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[[Category: M6P]]
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[[Category: NAG]]
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[[Category: OS]]
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[[Category: lectin; receptor; mannose 6-phosphate]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:51:41 2007''
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Current revision

N-terminal 3 domains of CI-MPR bound to mannose 6-phosphate

PDB ID 1sz0

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