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1sz7

From Proteopedia

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[[Image:1sz7.gif|left|200px]]<br /><applet load="1sz7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sz7, resolution 1.55&Aring;" />
 
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'''Crystal structure of Human Bet3'''<br />
 
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==Overview==
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==Crystal structure of Human Bet3==
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BET3 is a component of TRAPP, a complex involved in the tethering of transport vesicles to the cis-Golgi membrane. The crystal structure of human BET3 has been determined to 1.55-A resolution. BET3 adopts an alpha/beta-plait fold and forms dimers in the crystal and in solution, which predetermines the architecture of TRAPP where subunits are present in equimolar stoichiometry. A hydrophobic pocket within BET3 buries a palmitate bound through a thioester linkage to cysteine 68. BET3 and yeast Bet3p are palmitoylated in recombinant yeast cells, the mutant proteins BET3 C68S and Bet3p C80S remain unmodified. Both BET3 and BET3 C68S are found in membrane and cytosolic fractions of these cells; in membrane extractions, they behave like tightly membrane-associated proteins. In a deletion strain, both Bet3p and Bet3p C80S rescue cell viability. Thus, palmitoylation is neither required for viability nor sufficient for membrane association of BET3, which may depend on protein-protein contacts within TRAPP or additional, yet unidentified modifications of BET3. A conformational change may facilitate palmitoyl extrusion from BET3 and allow the fatty acid chain to engage in intermolecular hydrophobic interactions.
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<StructureSection load='1sz7' size='340' side='right'caption='[[1sz7]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1sz7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZ7 FirstGlance]. <br>
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1SZ7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=PLM:'>PLM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZ7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLM:PALMITIC+ACID'>PLM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sz7 OCA], [https://pdbe.org/1sz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sz7 RCSB], [https://www.ebi.ac.uk/pdbsum/1sz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sz7 ProSAT]</span></td></tr>
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Structure of palmitoylated BET3: insights into TRAPP complex assembly and membrane localization., Turnbull AP, Kummel D, Prinz B, Holz C, Schultchen J, Lang C, Niesen FH, Hofmann KP, Delbruck H, Behlke J, Muller EC, Jarosch E, Sommer T, Heinemann U, EMBO J. 2005 Mar 9;24(5):875-84. Epub 2005 Feb 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15692564 15692564]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TPPC3_HUMAN TPPC3_HUMAN] May play a role in vesicular transport from endoplasmic reticulum to Golgi.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1sz7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sz7 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Behlke, J.]]
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[[Category: Behlke J]]
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[[Category: Delbrueck, H.]]
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[[Category: Delbrueck H]]
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[[Category: Heinemann, U.]]
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[[Category: Heinemann U]]
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[[Category: Holz, C.]]
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[[Category: Holz C]]
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[[Category: Lang, C.]]
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[[Category: Lang C]]
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[[Category: Niesen, F H.]]
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[[Category: Niesen FH]]
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[[Category: Prinz, B.]]
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[[Category: Prinz B]]
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[[Category: Schultchen, J.]]
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[[Category: Schultchen J]]
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[[Category: Turnbull, A P.]]
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[[Category: Turnbull AP]]
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[[Category: PLM]]
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[[Category: alpha-beta plait]]
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[[Category: palmitoylated]]
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[[Category: trapp complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:07:55 2008''
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Current revision

Crystal structure of Human Bet3

PDB ID 1sz7

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