1szu

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(New page: 200px<br /><applet load="1szu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1szu, resolution 2.52&Aring;" /> '''The structure of gam...)
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[[Image:1szu.gif|left|200px]]<br /><applet load="1szu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1szu, resolution 2.52&Aring;" />
 
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'''The structure of gamma-aminobutyrate aminotransferase mutant: V241A'''<br />
 
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==Overview==
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==The structure of gamma-aminobutyrate aminotransferase mutant: V241A==
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The E. coli isozyme of gamma-aminobutyrate aminotransferase (GABA-AT) is a, tetrameric pyridoxal phosphate-dependent enzyme that catalyzes, transamination between primary amines and alpha-keto acids. The roles of, the active site residues V241, E211, and I50 in the GABA-AT mechanism have, been probed by site-directed mutagenesis. The beta-branched side chain of, V241 facilitates formation of external aldimine intermediates with primary, amine substrates, while E211 provides charge compensation of R398, selectively in the primary amine half-reaction and I50 forms a hydrophobic, lid at the top of the substrate binding site. The structures of the I50Q, V241A, and E211S mutants were solved by X-ray crystallography to, resolutions of 2.1, 2.5, and 2.52 A, respectively. The structure of, GABA-AT is similar in overall fold and active site structure to that of, dialkylglycine decarboxylase, which catalyzes both transamination and, decarboxylation half-reactions in its normal catalytic cycle. Therefore, an attempt was made to convert GABA-AT into a decarboxylation-dependent, aminotransferase similar to dialkylglycine decarboxylase by systematic, mutation of E. coli GABA-AT active site residues. Two of the twelve, mutants presented, E211S/I50G/C77K and E211S/I50H/V80D, have approximately, 10-fold higher decarboxylation activities than the wild-type enzyme, and, the E211S/I50H/V80D has formally changed the reaction specificity to that, of a decarboxylase.
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<StructureSection load='1szu' size='340' side='right'caption='[[1szu]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1szu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=PMP:4-DEOXY-4-AMINOPYRIDOXAL-5-PHOSPHATE'>PMP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szu OCA], [https://pdbe.org/1szu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szu RCSB], [https://www.ebi.ac.uk/pdbsum/1szu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GABT_ECOLI GABT_ECOLI] Catalyzes the transfer of the amino group from gamma-aminobutyrate (GABA) to alpha-ketoglutarate (KG) to yield succinic semialdehyde (SSA).<ref>PMID:20639325</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1szu ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SZU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with SO4, EDO, PLP and PMP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/4-aminobutyrate_transaminase 4-aminobutyrate transaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.19 2.6.1.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SZU OCA].
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*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Kinetic and crystallographic analysis of active site mutants of Escherichia coli gamma-aminobutyrate aminotransferase., Liu W, Peterson PE, Langston JA, Jin X, Zhou X, Fisher AJ, Toney MD, Biochemistry. 2005 Mar 1;44(8):2982-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15723541 15723541]
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__TOC__
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[[Category: 4-aminobutyrate transaminase]]
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Fisher, A.J.]]
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[[Category: Fisher AJ]]
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[[Category: Jin, X.]]
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[[Category: Jin X]]
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[[Category: Langston, J.A.]]
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[[Category: Langston JA]]
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[[Category: Liu, W.]]
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[[Category: Liu W]]
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[[Category: Peterson, P.E.]]
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[[Category: Peterson PE]]
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[[Category: Toney, M.D.]]
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[[Category: Toney MD]]
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[[Category: Zhou, X.]]
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[[Category: Zhou X]]
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[[Category: EDO]]
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[[Category: PLP]]
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[[Category: PMP]]
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[[Category: SO4]]
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[[Category: gaba-at]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:52:55 2007''
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Current revision

The structure of gamma-aminobutyrate aminotransferase mutant: V241A

PDB ID 1szu

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