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1t0h

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Crystal structure of the Rattus norvegicus voltage gated calcium channel beta subunit isoform 2a

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-[[Image:1t0h.gif|left|200px]]<br /><applet load="1t0h" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1t0h, resolution 1.97&Aring;" />
-'''Crystal structure of the Rattus norvegicus voltage gated calcium channel beta subunit isoform 2a'''<br />
-==Overview==
+==Crystal structure of the Rattus norvegicus voltage gated calcium channel beta subunit isoform 2a==
-Voltage-gated calcium channels (Ca(V)s) govern muscle contraction, hormone, and neurotransmitter release, neuronal migration, activation of, calcium-dependent signalling cascades, and synaptic input integration. An, essential Ca(V) intracellular protein, the beta-subunit (Ca(V)beta), binds, a conserved domain (the alpha-interaction domain, AID) between, transmembrane domains I and II of the pore-forming alpha(1) subunit and, profoundly affects multiple channel properties such as voltage-dependent, activation, inactivation rates, G-protein modulation, drug sensitivity and, cell surface expression. Here, we report the high-resolution crystal, structures of the Ca(V)beta2a conserved core, alone and in complex with, the AID. Previous work suggested that a conserved region, the, beta-interaction domain (BID), formed the AID-binding site; however, this, region is largely buried in the Ca(V)beta core and is unavailable for, protein-protein interactions. The structure of the AID-Ca(V)beta2a complex, shows instead that Ca(V)beta2a engages the AID through an extensive, conserved hydrophobic cleft (named the alpha-binding pocket, ABP). The, ABP-AID interaction positions one end of the Ca(V)beta near the, intracellular end of a pore-lining segment, called IS6, that has a, critical role in Ca(V) inactivation. Together, these data suggest that, Ca(V)betas influence Ca(V) gating by direct modulation of IS6 movement, within the channel pore.
+<StructureSection load='1t0h' size='340' side='right'caption='[[1t0h]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1t0h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T0H FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0h OCA], [https://pdbe.org/1t0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t0h RCSB], [https://www.ebi.ac.uk/pdbsum/1t0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0h ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/CACB2_RAT CACB2_RAT] The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting (By similarity).<ref>PMID:1370480</ref> <ref>PMID:11604404</ref> <ref>PMID:12042350</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t0/1t0h_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t0h ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-T0H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T0H OCA].
+*[[Ion channels 3D structures|Ion channels 3D structures]]
+== References ==
-==Reference==
+<references/>
-Structure of a complex between a voltage-gated calcium channel beta-subunit and an alpha-subunit domain., Van Petegem F, Clark KA, Chatelain FC, Minor DL Jr, Nature. 2004 Jun 10;429(6992):671-5. Epub 2004 May 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15141227 15141227]
+__TOC__
-[[Category: Protein complex]]
+</StructureSection>
+[[Category: Large Structures]]
 [[Category: Rattus norvegicus]]
-[[Category: Chatelain, F.]]
+[[Category: Chatelain F]]
-[[Category: Clark, K.]]
+[[Category: Clark K]]
-[[Category: Jr., D.Minor.]]
+[[Category: Minor Jr D]]
-[[Category: Petegem, F.Van.]]
+[[Category: Van Petegem F]]
-[[Category: CL]]
-[[Category: nucleotide kinase like domain]]
-[[Category: sh3 domain]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:53:41 2007''

1t0h

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Current revision

Crystal structure of the Rattus norvegicus voltage gated calcium channel beta subunit isoform 2a

Structural highlights

Function

Evolutionary Conservation

See Also

References

Contents

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