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1t2l

From Proteopedia

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[[Image:1t2l.jpg|left|200px]]<br /><applet load="1t2l" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t2l, resolution 2.8&Aring;" />
 
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'''Three Crystal Structures of Human Coactosin-like Protein'''<br />
 
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==Overview==
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==Three Crystal Structures of Human Coactosin-like Protein==
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Human coactosin-like protein is an actin filament binding protein but does, not bind to globular actin. It associates with 5-Lipoxygenase both in vivo, and in vitro, playing important roles in modulating the activities of, actin and 5-Lipoxygenase. Coactosin counteracts the capping activity of, capping protein which inhibits the actin polymerization. We determined the, crystal structures of human coactosin-like protein by multi-wavelength, anomalous dispersion method. The structure showed a high level of, similarity to ADF-H domain, although their amino acid sequences share low, degree of homology. A few conserved hydrophobic residues that may, contribute to the folding were identified. This structure suggests, coactosin-like protein bind to F-actin in a different way from ADF/Cofilin, family. Combined with the information from previous mutagenesis studies, the binding sites for F-actin and 5-Lipoxygenase were analyzed, respectively. These two sites are quite close, which might prevent F-actin, and 5-Lipoxygenase from binding to coactosin simultaneously.
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<StructureSection load='1t2l' size='340' side='right'caption='[[1t2l]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1t2l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2L FirstGlance]. <br>
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Known disease associated with this structure: Cardiomyopathy, dilated, 1M OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600824 600824]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2l OCA], [https://pdbe.org/1t2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2l RCSB], [https://www.ebi.ac.uk/pdbsum/1t2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2l ProSAT]</span></td></tr>
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1T2L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2L OCA].
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</table>
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== Function ==
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==Reference==
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[https://www.uniprot.org/uniprot/COTL1_HUMAN COTL1_HUMAN] Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization.<ref>PMID:11583571</ref>
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Crystal structure of human coactosin-like protein., Liu L, Wei Z, Wang Y, Wan M, Cheng Z, Gong W, J Mol Biol. 2004 Nov 19;344(2):317-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15522287 15522287]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2l ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chen, Z.]]
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[[Category: Chen Z]]
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[[Category: Gong, W.]]
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[[Category: Gong W]]
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[[Category: Liu, L.]]
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[[Category: Liu L]]
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[[Category: Wang, Y.]]
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[[Category: Wang Y]]
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[[Category: Wei, Z.]]
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[[Category: Wei Z]]
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[[Category: beta-sheet]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:55:00 2008''
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Current revision

Three Crystal Structures of Human Coactosin-like Protein

PDB ID 1t2l

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