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1t3t

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(New page: 200px<br /><applet load="1t3t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t3t, resolution 1.90&Aring;" /> '''Structure of Formylg...)
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[[Image:1t3t.gif|left|200px]]<br /><applet load="1t3t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t3t, resolution 1.90&Aring;" />
 
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'''Structure of Formylglycinamide synthetase'''<br />
 
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==Overview==
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==Structure of Formylglycinamide synthetase==
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Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the, ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and, glutamine to formylglycinamidine ribonucleotide (FGAM), ADP, P(i), and, glutamate in the fourth step of the purine biosynthetic pathway. In, eukaryotes and Gram-negative bacteria, FGAR-AT is encoded by the purL gene, as a multidomain protein with a molecular mass of about 140 kDa. In, Gram-positive bacteria and archaebacteria FGAR-AT is a complex of three, proteins: PurS, PurL, and PurQ. We have determined the structure of, FGAR-AT (PurL) from Salmonella typhimurium at 1.9 A resolution using X-ray, crystallography. PurL is the last remaining enzyme in the purine, biosynthetic pathway to have its structure determined. The structure, reveals four domains: an N-terminal domain structurally homologous to a, PurS dimer, a linker region, an FGAM synthetase domain homologous to an, aminoimidazole ribonucleotide synthetase (PurM) dimer, and a triad, glutaminase domain. The domains are intricately linked by interdomain, interactions and peptide connectors. The fold common to PurM and the, central region of PurL represents a superfamily for which HypE, SelD, and, ThiL are predicted to be members. A structural ADP molecule was found, bound to a site related to the putative active site by pseudo-2-fold, symmetry and two sulfate ions were found at the putative active site., These observations and the structural similarities between PurM and StPurL, were used to model the substrates FGAR and ATP in the StPurL active site., A glutamylthioester intermediate was found in the glutaminase domain at, Cys1135. The N-terminal (PurS-like) domain is hypothesized to form the, putative channel through which ammonia passes from the glutaminase domain, to the FGAM synthetase domain.
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<StructureSection load='1t3t' size='340' side='right'caption='[[1t3t]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1t3t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T3T FirstGlance]. <br>
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1T3T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with MG, SO4 and ADP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoribosylformylglycinamidine_synthase Phosphoribosylformylglycinamidine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.5.3 6.3.5.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T3T OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CYG:2-AMINO-4-(AMINO-3-OXO-PROPYLSULFANYLCARBONYL)-BUTYRIC+ACID'>CYG</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t3t OCA], [https://pdbe.org/1t3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t3t RCSB], [https://www.ebi.ac.uk/pdbsum/1t3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t3t ProSAT]</span></td></tr>
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Domain organization of Salmonella typhimurium formylglycinamide ribonucleotide amidotransferase revealed by X-ray crystallography., Anand R, Hoskins AA, Stubbe J, Ealick SE, Biochemistry. 2004 Aug 17;43(32):10328-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15301531 15301531]
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</table>
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[[Category: Phosphoribosylformylglycinamidine synthase]]
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== Function ==
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[[Category: Salmonella typhimurium]]
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[https://www.uniprot.org/uniprot/PUR4_SALTY PUR4_SALTY]
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Anand, R.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Ealick, S.E.]]
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Check<jmol>
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[[Category: Hoskin, A.A.]]
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<jmolCheckbox>
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[[Category: Stubbe, J.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t3/1t3t_consurf.spt"</scriptWhenChecked>
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[[Category: ADP]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: MG]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: SO4]]
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</jmolCheckbox>
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[[Category: fgam synthetase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t3t ConSurf].
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[[Category: formyl glycinamide]]
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<div style="clear:both"></div>
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[[Category: purl]]
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__TOC__
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[[Category: purq]]
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</StructureSection>
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[[Category: purs]]
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[[Category: Large Structures]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:58:05 2007''
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[[Category: Anand R]]
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[[Category: Ealick SE]]
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[[Category: Hoskin AA]]
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[[Category: Stubbe J]]

Current revision

Structure of Formylglycinamide synthetase

PDB ID 1t3t

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