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1t6r

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[[Image:1t6r.gif|left|200px]]
 
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==Solution structure of TM1442, a putative anti sigma factor antagonist in phosphorylated state==
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The line below this paragraph, containing "STRUCTURE_1t6r", creates the "Structure Box" on the page.
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<StructureSection load='1t6r' size='340' side='right'caption='[[1t6r]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1t6r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T6R FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
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{{STRUCTURE_1t6r| PDB=1t6r | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t6r OCA], [https://pdbe.org/1t6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t6r RCSB], [https://www.ebi.ac.uk/pdbsum/1t6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t6r ProSAT], [https://www.topsan.org/Proteins/JCSG/1t6r TOPSAN]</span></td></tr>
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</table>
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'''Solution structure of TM1442, a putative anti sigma factor antagonist in phosphorylated state'''
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== Function ==
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[https://www.uniprot.org/uniprot/Y1442_THEMA Y1442_THEMA] In the phosphorylated form it could act as an anti-anti-sigma factor that counteracts an anti-sigma factor and thus releases a sigma factor from inhibition (By similarity).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The NMR structures of the unphosphorylated Thermotoga maritima protein TM1442 at pH 4.8 and of the phosphorylated TM1442 (TM1442-P) at pH 7.0 are presented, and a functional interaction of TM1442 with TM0733 is characterized. Although the NMR spectra of TM1442-P at pH 7.0 are of high quality, detailed NMR studies of unphosphorylated TM1442 could be performed only at slightly acidic pH values and high salt concentration. TM1442 is a putative anti-sigma-factor antagonist related to the sigmaF and sigmaB regulation systems in Bacillus subtilis, which is the component in this system that can be phosphorylated. The kinase TM0733, which shows sequence similarity to the GHKL ATPase/kinase superfamily, was identified as the possible anti-sigma-factor of TM1442 using a bioinformatics analysis. Phosphorylation of TM1442 by TM0733 was confirmed by NMR, mass spectroscopy and native gel electrophoresis, and Ser59 was identified as the phosphorylation site using site-directed mutational analysis. The solution structure of TM1442-P at pH 7.0 has the same global fold as free TM1442 at pH 4.8, with an alpha/beta topology consisting of a central four-stranded beta sheet and three alpha helices, but the regular secondary structure elements wrapping the hydrophobic core of the protein undergo subtle conformational changes upon phosphorylation.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t6/1t6r_consurf.spt"</scriptWhenChecked>
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1T6R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6R OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Solution structures of the putative anti-sigma-factor antagonist TM1442 from Thermotoga maritima in the free and phosphorylated states., Etezady-Esfarjani T, Placzek WJ, Herrmann T, Wuthrich K, Magn Reson Chem. 2006 Jul;44 Spec No:S61-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16826544 16826544]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t6r ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Etezady-Esfarjani, T.]]
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[[Category: Etezady-Esfarjani T]]
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[[Category: Herrmann, T.]]
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[[Category: Herrmann T]]
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[[Category: JCSG, Joint Center for Structural Genomics.]]
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[[Category: Lesley SA]]
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[[Category: Lesley, S A.]]
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[[Category: Placzek W]]
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[[Category: Placzek, W.]]
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[[Category: Wuthrich K]]
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[[Category: Wuthrich, K.]]
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[[Category: Jcsg]]
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[[Category: Joint center for structural genomic]]
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[[Category: Phosphorylation]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Solution structure]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:36:04 2008''
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Current revision

Solution structure of TM1442, a putative anti sigma factor antagonist in phosphorylated state

PDB ID 1t6r

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