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1t8s

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(New page: 200px<br /><applet load="1t8s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t8s, resolution 2.60&Aring;" /> '''Crystal Structure of...)
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[[Image:1t8s.jpg|left|200px]]<br /><applet load="1t8s" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1t8s, resolution 2.60&Aring;" />
 
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'''Crystal Structure of E.coli AMP Nucleosidase complexed with formicin 5'-monophosphate'''<br />
 
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==Overview==
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==Crystal Structure of E.coli AMP Nucleosidase complexed with formicin 5'-monophosphate==
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AMP nucleosidase (AMN) catalyzes the hydrolysis of AMP to form adenine and, ribose 5-phosphate. The enzyme is found only in prokaryotes, where it, plays a role in purine nucleoside salvage and intracellular AMP level, regulation. Enzyme activity is stimulated by ATP and suppressed by, phosphate. The structure of unliganded AMN was determined at 2.7 A, resolution, and structures of the complexes with either formycin, 5'-monophosphate or inorganic phosphate were determined at 2.6 A and 3.0 A, resolution, respectively. AMN is a biological homohexamer, and each, monomer is composed of two domains: a catalytic domain and a putative, regulatory domain. The overall topology of the catalytic domain and some, features of the substrate binding site resemble those of the nucleoside, phosphorylases, demonstrating that AMN is a new member of the family. The, structure of the regulatory domain consists of a long helix and a, four-stranded sheet and has a novel topology.
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<StructureSection load='1t8s' size='340' side='right'caption='[[1t8s]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1t8s]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8S FirstGlance]. <br>
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1T8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with FMP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/AMP_nucleosidase AMP nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.4 3.2.2.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1T8S OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMP:FORMYCIN-5-MONOPHOSPHATE'>FMP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8s OCA], [https://pdbe.org/1t8s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8s RCSB], [https://www.ebi.ac.uk/pdbsum/1t8s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8s ProSAT]</span></td></tr>
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Structure of Escherichia coli AMP nucleosidase reveals similarity to nucleoside phosphorylases., Zhang Y, Cottet SE, Ealick SE, Structure. 2004 Aug;12(8):1383-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15296732 15296732]
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</table>
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[[Category: AMP nucleosidase]]
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== Function ==
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[https://www.uniprot.org/uniprot/AMN_ECOLI AMN_ECOLI] Involved in regulation of AMP concentrations.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8s_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t8s ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cottet, S.E.]]
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[[Category: Cottet SE]]
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[[Category: Ealick, S.E.]]
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[[Category: Ealick SE]]
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[[Category: Zhang, Y.]]
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[[Category: Zhang Y]]
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[[Category: FMP]]
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[[Category: alpha-beta fold]]
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[[Category: alpha-beta-alpha sandwich]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:04:47 2007''
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Current revision

Crystal Structure of E.coli AMP Nucleosidase complexed with formicin 5'-monophosphate

PDB ID 1t8s

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