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1ta3

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[[Image:1ta3.gif|left|200px]]
 
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==Crystal Structure of xylanase (GH10) in complex with inhibitor (XIP)==
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The line below this paragraph, containing "STRUCTURE_1ta3", creates the "Structure Box" on the page.
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<StructureSection load='1ta3' size='340' side='right'caption='[[1ta3]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ta3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans Aspergillus nidulans] and [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TA3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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{{STRUCTURE_1ta3| PDB=1ta3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ta3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ta3 OCA], [https://pdbe.org/1ta3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ta3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ta3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ta3 ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of xylanase (GH10) in complex with inhibitor (XIP)'''
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== Function ==
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[https://www.uniprot.org/uniprot/XIP1_WHEAT XIP1_WHEAT] Fungal xylanase inhibitor. Possesses competitive inhibiting activity against fungal endo-1,4-beta-D-xylanases belonging to glycoside hydrolase family 10 (GH10) and family 11 (GH11). Possesses also inhibitory activity towards barley alpha-amylases. Binding to xylanases or amylases is necessary for inhibition activity. May function in plant defense against secreted fungal pathogen xylanases. Is similar to class III chitinases, but does not exhibit chitinase activity.<ref>PMID:12023019</ref> <ref>PMID:10024521</ref> <ref>PMID:11955286</ref> <ref>PMID:12922177</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The xylanase inhibitor protein I (XIP-I) from wheat Triticum aestivum is the prototype of a novel class of cereal protein inhibitors that inhibit fungal xylanases belonging to glycoside hydrolase families 10 (GH10) and 11 (GH11). The crystal structures of XIP-I in complex with Aspergillus nidulans (GH10) and Penicillium funiculosum (GH11) xylanases have been solved at 1.7 and 2.5 A resolution, respectively. The inhibition strategy is novel because XIP-I possesses two independent enzyme-binding sites, allowing binding to two glycoside hydrolases that display a different fold. Inhibition of the GH11 xylanase is mediated by the insertion of an XIP-I Pi-shaped loop (Lalpha(4)beta(5)) into the enzyme active site, whereas residues in the helix alpha7 of XIP-I, pointing into the four central active site subsites, are mainly responsible for the reversible inactivation of GH10 xylanases. The XIP-I strategy for inhibition of xylanases involves substrate-mimetic contacts and interactions occluding the active site. The structural determinants of XIP-I specificity demonstrate that the inhibitor is able to interact with GH10 and GH11 xylanases of both fungal and bacterial origin. The biological role of the xylanase inhibitors is discussed in light of the present structural data.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ta/1ta3_consurf.spt"</scriptWhenChecked>
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1TA3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans] and [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TA3 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The dual nature of the wheat xylanase protein inhibitor XIP-I: structural basis for the inhibition of family 10 and family 11 xylanases., Payan F, Leone P, Porciero S, Furniss C, Tahir T, Williamson G, Durand A, Manzanares P, Gilbert HJ, Juge N, Roussel A, J Biol Chem. 2004 Aug 20;279(34):36029-37. Epub 2004 Jun 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15181003 15181003]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ta3 ConSurf].
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[[Category: Emericella nidulans]]
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<div style="clear:both"></div>
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[[Category: Endo-1,4-beta-xylanase]]
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== References ==
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[[Category: Protein complex]]
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Aspergillus nidulans]]
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[[Category: Large Structures]]
[[Category: Triticum aestivum]]
[[Category: Triticum aestivum]]
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[[Category: Durand, A.]]
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[[Category: Durand A]]
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[[Category: Furniss, C.]]
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[[Category: Furniss C]]
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[[Category: Gilbert, H J.]]
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[[Category: Gilbert HJ]]
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[[Category: Juge, N.]]
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[[Category: Juge N]]
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[[Category: Leone, P.]]
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[[Category: Leone P]]
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[[Category: Manzanares, P.]]
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[[Category: Manzanares P]]
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[[Category: Payan, F.]]
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[[Category: Payan F]]
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[[Category: Porciero, S.]]
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[[Category: Porciero S]]
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[[Category: Roussel, A.]]
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[[Category: Roussel A]]
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[[Category: Tahir, T.]]
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[[Category: Tahir T]]
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[[Category: Williamson, G.]]
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[[Category: Williamson G]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:43:45 2008''
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Current revision

Crystal Structure of xylanase (GH10) in complex with inhibitor (XIP)

PDB ID 1ta3

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