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1tew

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(New page: 200px<br /><applet load="1tew" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tew, resolution 1.65&Aring;" /> '''STRUCTURE OF HEXAGON...)
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[[Image:1tew.jpg|left|200px]]<br /><applet load="1tew" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tew, resolution 1.65&Aring;" />
 
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'''STRUCTURE OF HEXAGONAL TURKEY EGG WHITE LYSOZYME AT 1.65 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==STRUCTURE OF HEXAGONAL TURKEY EGG WHITE LYSOZYME AT 1.65 ANGSTROMS RESOLUTION==
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The structure of hexagonal turkey egg-white lysozyme (TEWL) has been, determined and refined at 1.65 A resolution. The crystals were grown from, a 150 mM potassium thiocyanate solution at pH 4.5 and belong to space, group P6(1)22 with unit-cell dimensions a = b = 70.96, c = 83.01 A alpha =, beta = 90, gamma = 120 degrees. The crystals were isomorphous with those, of hexagonal pH 8.0 TEWL. The coordinates of PDB entry code 3LZ2 were, therefore used as the initial model and subjected to rigid-body, refinement, simulated annealing and least-squares refinement to a final, residual of 0.20. The root-mean-square deviations from the ideal bond, distances and angles were 0.016 A and 2.2 degrees, respectively. During, the refinement, 86 water molecules and one thiocyanate ion were located in, the structure. The thiocyanate ion lies close to the interface between two, symmetry-related molecules. The S atom of the ion forms two direct, intermolecular contacts with Argl4 and interacts indirectly via a network, of water molecules to Arg5 of a symmetry-related molecule. The structure, provides direct evidence for the mode of thiocyanate binding to arginine, residues and suggests a possible mechanism for the efficiency of, thiocyanate in crystallizing basic proteins.
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<StructureSection load='1tew' size='340' side='right'caption='[[1tew]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tew]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TEW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TEW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tew FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tew OCA], [https://pdbe.org/1tew PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tew RCSB], [https://www.ebi.ac.uk/pdbsum/1tew PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tew ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYSC_MELGA LYSC_MELGA] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1tew_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tew ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TEW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo] with SCN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TEW OCA].
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of hexagonal turkey egg-white lysozyme at 1.65A resolution., Howell PL, Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):654-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299795 15299795]
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[[Category: Large Structures]]
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[[Category: Lysozyme]]
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[[Category: Meleagris gallopavo]]
[[Category: Meleagris gallopavo]]
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[[Category: Single protein]]
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[[Category: Howell PL]]
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[[Category: Howell, P.L.]]
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[[Category: SCN]]
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[[Category: hydrolase(o-glycosyl)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:12:32 2007''
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Current revision

STRUCTURE OF HEXAGONAL TURKEY EGG WHITE LYSOZYME AT 1.65 ANGSTROMS RESOLUTION

PDB ID 1tew

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