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1tih

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(New page: 200px<br /><applet load="1tih" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tih" /> '''TRYPSIN INHIBITOR (T1) FROM NICOTIANA ALATA'...)
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[[Image:1tih.jpg|left|200px]]<br /><applet load="1tih" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1tih" />
 
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'''TRYPSIN INHIBITOR (T1) FROM NICOTIANA ALATA'''<br />
 
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==Overview==
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==TRYPSIN INHIBITOR (T1) FROM NICOTIANA ALATA==
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The three-dimensional structures of a series of 6-kDa trypsin inhibitors, isolated from the stigma of the ornamental tobacco Nicotiana alata have, been determined by 1H NMR spectroscopy combined with simulated annealing, calculations. The proteins, T1-T4, are proteolytically cleaved from a, 40.3-kDa precursor protein, NA-proPI, together with a chymotrypsin, inhibitor, C1, the structure of which was reported recently [Nielsen, K.J., Heath, R.L., Anderson, M.A., &amp; Craik, D.J. (1994) J. Mol. Biol. 242, 231-243]. Each of the proteinase inhibitors comprises 53 amino acids, including 8 cysteine residues which are linked to form 4 disulfide, bridges. The proteins have a high degree of sequence identity and differ, mainly in residues around the putative reactive sites. The structure of T1, was determined using a set of 533 interproton distance restraints derived, from NOESY spectra, combined with 33 dihedral restraints derived from, 3JNH-H alpha coupling constants and 16 hydrogen bonds. The structures of, the remaining inhibitors (T2-T4) were deduced to be almost identical to, T1, on the basis of their similar chemical shifts and 2D spectra. The, current study demonstrates that the structures of the trypsin inhibitors, (T1-T4) are similar to that previously found for the chymotrypsin, inhibitor, C1. Despite differences in sequence, there is conservation in, backbone geometry between the reactive site loops of the two classes of, inhibitors. From this, it is clear that the nature of the side chain on, the primary binding residue, rather than the backbone fold, is the main, determinant of the enzyme specificities of these proteinase inhibitors.
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<StructureSection load='1tih' size='340' side='right'caption='[[1tih]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1tih]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_alata Nicotiana alata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TIH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tih OCA], [https://pdbe.org/1tih PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tih RCSB], [https://www.ebi.ac.uk/pdbsum/1tih PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tih ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q40378_NICAL Q40378_NICAL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ti/1tih_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tih ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1TIH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nicotiana_alata Nicotiana alata]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TIH OCA].
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*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structures of a series of 6-kDa trypsin inhibitors isolated from the stigma of Nicotiana alata., Nielsen KJ, Heath RL, Anderson MA, Craik DJ, Biochemistry. 1995 Nov 7;34(44):14304-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7578034 7578034]
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[[Category: Large Structures]]
[[Category: Nicotiana alata]]
[[Category: Nicotiana alata]]
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[[Category: Single protein]]
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[[Category: Anderson MA]]
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[[Category: Anderson, M.A.]]
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[[Category: Craik DJ]]
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[[Category: Craik, D.J.]]
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[[Category: Heath RL]]
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[[Category: Heath, R.L.]]
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[[Category: Nielsen KJ]]
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[[Category: Nielsen, K.J.]]
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[[Category: nicotiana alata trypsin inhibitor]]
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[[Category: potato ii trypsin inhibitor]]
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[[Category: serine proteinase inhibitor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 02:15:12 2007''
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Current revision

TRYPSIN INHIBITOR (T1) FROM NICOTIANA ALATA

PDB ID 1tih

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