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1tl8

From Proteopedia

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[[Image:1tl8.png|left|200px]]
 
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==Human DNA topoisomerase I (70 kDa) in complex with the indenoisoquinoline AI-III-52 and covalent complex with a 22 base pair DNA duplex==
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The line below this paragraph, containing "STRUCTURE_1tl8", creates the "Structure Box" on the page.
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<StructureSection load='1tl8' size='340' side='right'caption='[[1tl8]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1tl8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TL8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AI3:2,3-DIMETHOXY-12H-[1,3]DIOXOLO[5,6]INDENO[1,2-C]ISOQUINOLIN-6-IUM'>AI3</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene>, <scene name='pdbligand=TPC:5-THIO-2-DEOXY-CYTOSINE+PHOSPHONIC+ACID'>TPC</scene></td></tr>
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{{STRUCTURE_1tl8| PDB=1tl8 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tl8 OCA], [https://pdbe.org/1tl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tl8 RCSB], [https://www.ebi.ac.uk/pdbsum/1tl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tl8 ProSAT]</span></td></tr>
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</table>
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===Human DNA topoisomerase I (70 kDa) in complex with the indenoisoquinoline AI-III-52 and covalent complex with a 22 base pair DNA duplex===
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== Disease ==
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[https://www.uniprot.org/uniprot/TOP1_HUMAN TOP1_HUMAN] Note=A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
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== Function ==
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[https://www.uniprot.org/uniprot/TOP1_HUMAN TOP1_HUMAN] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.<ref>PMID:2833744</ref> <ref>PMID:19168442</ref> <ref>PMID:14594810</ref> <ref>PMID:16033260</ref>
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The line below this paragraph, {{ABSTRACT_PUBMED_16033260}}, adds the Publication Abstract to the page
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== Evolutionary Conservation ==
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(as it appears on PubMed at http://www.pubmed.gov), where 16033260 is the PubMed ID number.
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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{{ABSTRACT_PUBMED_16033260}}
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tl/1tl8_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[1tl8]] is a 4 chain structure of [[Topoisomerase]] with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TL8 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tl8 ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
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*[[Topoisomerase]]
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*[[Topoisomerase 3D structures|Topoisomerase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:16033260</ref><references group="xtra"/>
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__TOC__
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[[Category: DNA topoisomerase]]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Antony, S.]]
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[[Category: Large Structures]]
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[[Category: Cushman, M.]]
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[[Category: Antony S]]
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[[Category: Ioanoviciu, A.]]
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[[Category: Cushman M]]
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[[Category: Pommier, Y.]]
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[[Category: Ioanoviciu A]]
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[[Category: Staker, B L.]]
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[[Category: Pommier Y]]
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[[Category: Stewart, L.]]
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[[Category: Staker BL]]
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[[Category: Dna]]
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[[Category: Stewart L]]
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[[Category: Drug]]
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[[Category: Idenoisoquinoline]]
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[[Category: Poison]]
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[[Category: Topoisomerase i]]
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Current revision

Human DNA topoisomerase I (70 kDa) in complex with the indenoisoquinoline AI-III-52 and covalent complex with a 22 base pair DNA duplex

PDB ID 1tl8

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