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1tm2

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[[Image:1tm2.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of the apo form of the Salmonella typhimurium AI-2 receptor LsrB==
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|PDB= 1tm2 |SIZE=350|CAPTION= <scene name='initialview01'>1tm2</scene>, resolution 1.9&Aring;
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<StructureSection load='1tm2' size='340' side='right'caption='[[1tm2]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[1tm2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TM2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TM2 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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|GENE= LsrB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 Salmonella typhimurium LT2])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tm2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tm2 OCA], [https://pdbe.org/1tm2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tm2 RCSB], [https://www.ebi.ac.uk/pdbsum/1tm2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tm2 ProSAT]</span></td></tr>
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}}
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</table>
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== Function ==
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'''Crystal Structure of the apo form of the Salmonella typhimurium AI-2 receptor LsrB'''
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[https://www.uniprot.org/uniprot/LSRB_SALTY LSRB_SALTY] Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Binds AI-2 and delivers it to the LsrC and LsrD permeases (Probable).<ref>PMID:11722742</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Bacterial populations use cell-cell communication to coordinate community-wide regulation of processes such as biofilm formation, virulence, and bioluminescence. This phenomenon, termed quorum sensing, is mediated by small molecule signals known as autoinducers. While most autoinducers are species specific, autoinducer-2 (AI-2), first identified in the marine bacterium Vibrio harveyi, is produced and detected by many Gram-negative and Gram-positive bacteria. The crystal structure of the V. harveyi AI-2 signaling molecule bound to its receptor protein revealed an unusual furanosyl borate diester. Here, we present the crystal structure of a second AI-2 signal binding protein, LsrB from Salmonella typhimurium. We find that LsrB binds a chemically distinct form of the AI-2 signal, (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF), that lacks boron. Our results demonstrate that two different species of bacteria recognize two different forms of the autoinducer signal, both derived from 4,5-dihydroxy-2,3-pentanedione (DPD), and reveal new sophistication in the chemical lexicon used by bacteria in interspecies signaling.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tm/1tm2_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1TM2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TM2 OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tm2 ConSurf].
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Salmonella typhimurium recognizes a chemically distinct form of the bacterial quorum-sensing signal AI-2., Miller ST, Xavier KB, Campagna SR, Taga ME, Semmelhack MF, Bassler BL, Hughson FM, Mol Cell. 2004 Sep 10;15(5):677-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15350213 15350213]
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<div style="clear:both"></div>
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[[Category: Salmonella typhimurium lt2]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Bassler, B L.]]
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__TOC__
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[[Category: Campagna, S R.]]
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</StructureSection>
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[[Category: Hughson, F M.]]
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[[Category: Large Structures]]
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[[Category: Miller, S T.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
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[[Category: Semmelhack, M F.]]
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[[Category: Bassler BL]]
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[[Category: Taga, M E.]]
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[[Category: Campagna SR]]
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[[Category: Xavier, K B.]]
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[[Category: Hughson FM]]
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[[Category: periplasmic binding protein]]
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[[Category: Miller ST]]
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[[Category: Semmelhack MF]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:20:17 2008''
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[[Category: Taga ME]]
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[[Category: Xavier KB]]

Current revision

Crystal Structure of the apo form of the Salmonella typhimurium AI-2 receptor LsrB

PDB ID 1tm2

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