This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1to4

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1to4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1to4, resolution 1.55&Aring;" /> '''Structure of the cyt...)
Current revision (08:42, 1 May 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1to4.gif|left|200px]]<br /><applet load="1to4" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1to4, resolution 1.55&Aring;" />
 
-
'''Structure of the cytosolic Cu,Zn SOD from S. mansoni'''<br />
 
-
==Overview==
+
==Structure of the cytosolic Cu,Zn SOD from S. mansoni==
-
Cu,Zn superoxide dismutase (Cu,Zn SOD) is an essential enzyme for, protecting cells from the toxic effects of reactive oxygen species. In, humans, two distinct Cu,Zn SOD genes are located on chromosomes 4 and 21, and mutations in the latter have been associated with familial amyotrophic, lateral sclerosis. Similarly, schistosomes (trematode parasites, responsible for the chronically debilitating disease schistosomiasis) also, produce two distinct Cu,Zn SODs, in this case one cytosolic and one, bearing a signal peptide. The crystal structure of the cytosolic form of, the enzyme from the human trematode Schistosoma mansoni (SmCtSOD) was, solved and refined to a resolution of 2.2 A (space group P2(1)2(1)2(1), R, = 17.6% and R(free) = 24.1%) and 1.55 A (space group P2(1), R = 15.7% and, R(free) = 17.1%). This is the first report of a crystal structure of a, Cu,Zn superoxide dismutase derived from a human parasite. Alternate, positions for the catalytic copper and its water ligand were refined for, the 1.55 A SmCtSOD model, but the most interesting structural differences, between SmCtSOD and the human homologue reside in the loops used for, electrostatic guidance of the substrate to the enzyme active site.
+
<StructureSection load='1to4' size='340' side='right'caption='[[1to4]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1to4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TO4 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1to4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1to4 OCA], [https://pdbe.org/1to4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1to4 RCSB], [https://www.ebi.ac.uk/pdbsum/1to4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1to4 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/SODC_SCHMA SODC_SCHMA] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/to/1to4_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1to4 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1TO4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schistosoma_mansoni Schistosoma mansoni] with CU and ZN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TO4 OCA].
+
*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structure of the cytosolic Cu,Zn superoxide dismutase from Schistosoma mansoni., Cardoso RM, Silva CH, Ulian de Araujo AP, Tanaka T, Tanaka M, Garratt RC, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1569-78. Epub 2004, Aug 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15333927 15333927]
+
[[Category: Large Structures]]
[[Category: Schistosoma mansoni]]
[[Category: Schistosoma mansoni]]
-
[[Category: Single protein]]
+
[[Category: Cardoso RMF]]
-
[[Category: Superoxide dismutase]]
+
[[Category: Garratt RC]]
-
[[Category: Araujo, A.P.Ulian.de.]]
+
[[Category: Silva CHTP]]
-
[[Category: Cardoso, R.M.F.]]
+
[[Category: Tanaka M]]
-
[[Category: Garratt, R.C.]]
+
[[Category: Tanaka T]]
-
[[Category: Silva, C.H.T.P.]]
+
[[Category: Ulian de Araujo AP]]
-
[[Category: Tanaka, M.]]
+
-
[[Category: Tanaka, T.]]
+
-
[[Category: CU]]
+
-
[[Category: ZN]]
+
-
[[Category: beta-barrel]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:27:20 2007''
+

Current revision

Structure of the cytosolic Cu,Zn SOD from S. mansoni

PDB ID 1to4

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1to4"
Personal tools