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1u04

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[[Image:1u04.gif|left|200px]]
 
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{{Structure
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==Crystal structure of full length Argonaute from Pyrococcus furiosus==
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|PDB= 1u04 |SIZE=350|CAPTION= <scene name='initialview01'>1u04</scene>, resolution 2.25&Aring;
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<StructureSection load='1u04' size='340' side='right'caption='[[1u04]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
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<table><tr><td colspan='2'>[[1u04]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. The February 2008 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Small Interfering RNA'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2008_2 10.2210/rcsb_pdb/mom_2008_2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U04 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U04 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u04 OCA], [https://pdbe.org/1u04 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u04 RCSB], [https://www.ebi.ac.uk/pdbsum/1u04 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u04 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u04 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u04 OCA], [http://www.ebi.ac.uk/pdbsum/1u04 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u04 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q8U3D2_PYRFU Q8U3D2_PYRFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u04_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u04 ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of full length Argonaute from Pyrococcus furiosus'''
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==See Also==
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*[[Argonaute 3D structures|Argonaute 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Argonaute proteins and small interfering RNAs (siRNAs) are the known signature components of the RNA interference effector complex RNA-induced silencing complex (RISC). However, the identity of "Slicer," the enzyme that cleaves the messenger RNA (mRNA) as directed by the siRNA, has not been resolved. Here, we report the crystal structure of the Argonaute protein from Pyrococcus furiosus at 2.25 angstrom resolution. The structure reveals a crescent-shaped base made up of the amino-terminal, middle, and PIWI domains. The Piwi Argonaute Zwille (PAZ) domain is held above the base by a "stalk"-like region. The PIWI domain (named for the protein piwi) is similar to ribonuclease H, with a conserved active site aspartate-aspartate-glutamate motif, strongly implicating Argonaute as "Slicer." The architecture of the molecule and the placement of the PAZ and PIWI domains define a groove for substrate binding and suggest a mechanism for siRNA-guided mRNA cleavage.
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[[Category: Large Structures]]
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[[Category: Pyrococcus furiosus DSM 3638]]
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==About this Structure==
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[[Category: RCSB PDB Molecule of the Month]]
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1U04 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U04 OCA].
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[[Category: Small Interfering RNA]]
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[[Category: Hannon GJ]]
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==Reference==
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[[Category: Joshua-Tor L]]
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Crystal structure of Argonaute and its implications for RISC slicer activity., Song JJ, Smith SK, Hannon GJ, Joshua-Tor L, Science. 2004 Sep 3;305(5689):1434-7. Epub 2004 Jul 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15284453 15284453]
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[[Category: Smith SK]]
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[[Category: Pyrococcus furiosus dsm 3638]]
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[[Category: Song JJ]]
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[[Category: Single protein]]
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[[Category: Hannon, G J.]]
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[[Category: Joshua-Tor, L.]]
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[[Category: Smith, S K.]]
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[[Category: Song, J J.]]
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[[Category: argonaute]]
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[[Category: paz]]
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[[Category: piwi]]
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[[Category: risc]]
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[[Category: rnai]]
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[[Category: rnase h]]
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[[Category: silencing]]
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[[Category: slicer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:30 2008''
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Current revision

Crystal structure of full length Argonaute from Pyrococcus furiosus

PDB ID 1u04

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