This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1u24

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1u24" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u24, resolution 2.0&Aring;" /> '''Crystal structure of ...)
Current revision (08:46, 1 May 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1u24.gif|left|200px]]<br /><applet load="1u24" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1u24, resolution 2.0&Aring;" />
 
-
'''Crystal structure of Selenomonas ruminantium phytase'''<br />
 
-
==Overview==
+
==Crystal structure of Selenomonas ruminantium phytase==
-
Various inositide phosphatases participate in the regulation of inositol, polyphosphate signaling molecules. Plant phytases are phosphatases that, hydrolyze phytate to less-phosphorylated myo-inositol derivatives and, phosphate. The phytase from Selenomonas ruminantium shares no sequence, homology with other microbial phytases. Its crystal structure revealed a, phytase fold of the dual-specificity phosphatase type. The active site is, located near a conserved cysteine-containing (Cys241) P loop. We also, solved two other crystal forms in which an inhibitor, myo-inositol, hexasulfate, is cocrystallized with the enzyme. In the "standby" and the, "inhibited" crystal forms, the inhibitor is bound, respectively, in a, pocket slightly away from Cys241 and at the substrate binding site where, the phosphate group to be hydrolyzed is held close to the -SH group of, Cys241. Our structural and mutagenesis studies allow us to visualize the, way in which the P loop-containing phytase attracts and hydrolyzes the, substrate (phytate) sequentially.
+
<StructureSection load='1u24' size='340' side='right'caption='[[1u24]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1u24]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U24 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U24 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u24 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u24 OCA], [https://pdbe.org/1u24 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u24 RCSB], [https://www.ebi.ac.uk/pdbsum/1u24 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u24 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q7WUJ1_SELRU Q7WUJ1_SELRU]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u2/1u24_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u24 ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1U24 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Selenomonas_ruminantium Selenomonas ruminantium]. Active as [http://en.wikipedia.org/wiki/5-phytase 5-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.72 3.1.3.72] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U24 OCA].
+
*[[Phytase 3D structures|Phytase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Structures of Selenomonas ruminantium phytase in complex with persulfated phytate: DSP phytase fold and mechanism for sequential substrate hydrolysis., Chu HM, Guo RT, Lin TW, Chou CC, Shr HL, Lai HL, Tang TY, Cheng KJ, Selinger BL, Wang AH, Structure. 2004 Nov;12(11):2015-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15530366 15530366]
+
[[Category: Large Structures]]
-
[[Category: 5-phytase]]
+
[[Category: Selenomonas ruminantium]]
[[Category: Selenomonas ruminantium]]
-
[[Category: Single protein]]
+
[[Category: Cheng KJ]]
-
[[Category: Cheng, K.J.]]
+
[[Category: Chou CC]]
-
[[Category: Chou, C.C.]]
+
[[Category: Chu HM]]
-
[[Category: Chu, H.M.]]
+
[[Category: Guo RT]]
-
[[Category: Guo, R.T.]]
+
[[Category: Lai HL]]
-
[[Category: Lai, H.L.]]
+
[[Category: Lin TW]]
-
[[Category: Lin, T.W.]]
+
[[Category: Selinger BL]]
-
[[Category: Selinger, B.L.]]
+
[[Category: Shr HL]]
-
[[Category: Shr, H.L.]]
+
[[Category: Tang TY]]
-
[[Category: Tang, T.Y.]]
+
[[Category: Wang AH-J]]
-
[[Category: Wang, A.H.J.]]
+
-
[[Category: p-loop]]
+
-
[[Category: phytase]]
+
-
[[Category: ptp]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:10:31 2007''
+

Current revision

Crystal structure of Selenomonas ruminantium phytase

PDB ID 1u24

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1u24"
Personal tools