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1u34

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(New page: 200px<br /><applet load="1u34" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u34" /> '''3D NMR structure of the first extracellular ...)
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[[Image:1u34.gif|left|200px]]<br /><applet load="1u34" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u34" />
 
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'''3D NMR structure of the first extracellular domain of CRFR-2beta, a type B1 G-protein coupled receptor'''<br />
 
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==Overview==
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==3D NMR structure of the first extracellular domain of CRFR-2beta, a type B1 G-protein coupled receptor==
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The corticotropin-releasing factor (CRF) ligand family has diverse effects, on the CNS, including the modulation of the stress response. The ligands', effects are mediated by binding to CRF G protein-coupled receptors. We, have determined the 3D NMR structure of the N-terminal extracellular, domain (ECD1) of the mouse CRF receptor 2beta, which is the major ligand, recognition domain, and identified its ligand binding site by, chemical-shift perturbation experiments. The fold is identified as a short, consensus repeat (SCR), a common protein interaction module. Mutagenesis, reveals the integrity of the hormone-binding site in the full-length, receptor. This study proposes that the ECD1 captures the C-terminal, segment of the ligand, whose N terminus then penetrates into the, transmembrane region of the receptor to initiate signaling. Key residues, of SCR in the ECD1 are conserved in the G protein-coupled receptor, subfamily, suggesting the SCR fold in all of the ECD1s of this subfamily.
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<StructureSection load='1u34' size='340' side='right'caption='[[1u34]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1u34]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U34 FirstGlance]. <br>
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1U34 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U34 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u34 OCA], [https://pdbe.org/1u34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u34 RCSB], [https://www.ebi.ac.uk/pdbsum/1u34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u34 ProSAT]</span></td></tr>
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==Reference==
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</table>
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NMR structure and peptide hormone binding site of the first extracellular domain of a type B1 G protein-coupled receptor., Grace CR, Perrin MH, DiGruccio MR, Miller CL, Rivier JE, Vale WW, Riek R, Proc Natl Acad Sci U S A. 2004 Aug 31;101(35):12836-41. Epub 2004 Aug 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15326300 15326300]
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== Function ==
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[https://www.uniprot.org/uniprot/CRFR2_MOUSE CRFR2_MOUSE] This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u3/1u34_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u34 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: DiGruccio MR]]
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[[Category: DiGruccio, M.R.]]
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[[Category: Grace CR]]
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[[Category: Grace, C.R.]]
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[[Category: Miller CL]]
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[[Category: Miller, C.L.]]
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[[Category: Perrin MH]]
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[[Category: Perrin, M.H.]]
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[[Category: Riek R]]
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[[Category: Riek, R.]]
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[[Category: Rivier JE]]
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[[Category: Rivier, J.E.]]
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[[Category: Vale WW]]
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[[Category: Vale, W.W.]]
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[[Category: beta sheets and loops]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 03:48:54 2007''
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Current revision

3D NMR structure of the first extracellular domain of CRFR-2beta, a type B1 G-protein coupled receptor

PDB ID 1u34

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