This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
7ou1
From Proteopedia
(Difference between revisions)
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
==Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MP2)== | ==Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MP2)== | ||
| - | <StructureSection load='7ou1' size='340' side='right'caption='[[7ou1]]' scene=''> | + | <StructureSection load='7ou1' size='340' side='right'caption='[[7ou1]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OU1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ou1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhizobium_etli_CFN_42 Rhizobium etli CFN 42]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OU1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OU1 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ou1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ou1 OCA], [https://pdbe.org/7ou1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ou1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ou1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ou1 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ou1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ou1 OCA], [https://pdbe.org/7ou1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ou1 RCSB], [https://www.ebi.ac.uk/pdbsum/7ou1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ou1 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q2K0Z2_RHIEC Q2K0Z2_RHIEC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Rhizobium etli, a nitrogen-fixing bacterial symbiont of legume plants, encodes an essential L-asparaginase (ReAV) with no sequence homology to known enzymes with this activity. High-resolution crystal structures of ReAV show indeed a structurally distinct, dimeric enzyme, with some resemblance to glutaminases and beta-lactamases. However, ReAV has no glutaminase or lactamase activity, and at pH 9 its allosteric asparaginase activity is relatively high, with Km for L-Asn at 4.2 mM and kcat of 438 s(-1). The active site of ReAV, deduced from structural comparisons and confirmed by mutagenesis experiments, contains a highly specific Zn(2+) binding site without a catalytic role. The extensive active site includes residues with unusual chemical properties. There are two Ser-Lys tandems, all connected through a network of H-bonds to the Zn center, and three tightly bound water molecules near Ser48, which clearly indicate the catalytic nucleophile. | ||
| + | |||
| + | Crystal structures of the elusive Rhizobium etli L-asparaginase reveal a peculiar active site.,Loch JI, Imiolczyk B, Sliwiak J, Wantuch A, Bejger M, Gilski M, Jaskolski M Nat Commun. 2021 Nov 18;12(1):6717. doi: 10.1038/s41467-021-27105-x. PMID:34795296<ref>PMID:34795296</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7ou1" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Asparaginase 3D structures|Asparaginase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Rhizobium etli CFN 42]] | ||
[[Category: Gilski M]] | [[Category: Gilski M]] | ||
[[Category: Imiolczyk B]] | [[Category: Imiolczyk B]] | ||
[[Category: Jaskolski M]] | [[Category: Jaskolski M]] | ||
[[Category: Loch JI]] | [[Category: Loch JI]] | ||
Current revision
Crystal structure of Rhizobium etli inducible L-asparaginase ReAV (monoclinic form MP2)
| |||||||||||
