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| | <StructureSection load='1e2t' size='340' side='right'caption='[[1e2t]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='1e2t' size='340' side='right'caption='[[1e2t]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1e2t]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E2T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1E2T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1e2t]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E2T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E2T FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-hydroxyarylamine_O-acetyltransferase N-hydroxyarylamine O-acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.118 2.3.1.118] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e2t OCA], [http://pdbe.org/1e2t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1e2t RCSB], [http://www.ebi.ac.uk/pdbsum/1e2t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1e2t ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e2t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e2t OCA], [https://pdbe.org/1e2t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e2t RCSB], [https://www.ebi.ac.uk/pdbsum/1e2t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e2t ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/NHOA_SALTY NHOA_SALTY] Catalyzes both the acetyl-CoA-dependent N-acetylation of aromatic amines and the O-acetylation of N-hydroxyarylamines. In vitro, catalyzes the O-acetylation of N-hydroxy-Glu-P-1, and the N-acetylation of isoniazid and 2-aminofluorene.<ref>PMID:1569093</ref> <ref>PMID:7889864</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: N-hydroxyarylamine O-acetyltransferase]] | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] |
| - | [[Category: Delgoda, R]] | + | [[Category: Delgoda R]] |
| - | [[Category: Noble, M E.M]] | + | [[Category: Noble MEM]] |
| - | [[Category: Sandy, J]] | + | [[Category: Sandy J]] |
| - | [[Category: Sim, E]] | + | [[Category: Sim E]] |
| - | [[Category: Sinclair, J C]] | + | [[Category: Sinclair JC]] |
| - | [[Category: Acetyl coa dependent]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
NHOA_SALTY Catalyzes both the acetyl-CoA-dependent N-acetylation of aromatic amines and the O-acetylation of N-hydroxyarylamines. In vitro, catalyzes the O-acetylation of N-hydroxy-Glu-P-1, and the N-acetylation of isoniazid and 2-aminofluorene.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Enzymes of the arylamine N-acetyltransferase (NAT) family are found in species ranging from Escherichia coli to humans. In humans they are known to be responsible for the acetylation of a number of arylamine and hydrazine drugs, and they are strongly linked to the carcinogenic potentiation of certain foreign substances. In prokaryotes their substrate specificities may vary and members of the gene family have been linked to pathways including amide synthesis during rifamycin production. Here we report the crystal structure at 2.8 A resolution of a representative member of this family from Salmonella typhimurium in the presence and absence of a covalently bound product analog. The structure reveals surprising mechanistic information including the presence of a Cys-His-Asp catalytic triad. The fold can be described in terms of three domains of roughly equal length with the second and third domains linked by an interdomain helix. The first two domains, a helical bundle and a beta-barrel, make up the catalytic triad using a structural motif identical to that of the cysteine protease superfamily.
Structure of arylamine N-acetyltransferase reveals a catalytic triad.,Sinclair JC, Sandy J, Delgoda R, Sim E, Noble ME Nat Struct Biol. 2000 Jul;7(7):560-4. PMID:10876241[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Watanabe M, Sofuni T, Nohmi T. Involvement of Cys69 residue in the catalytic mechanism of N-hydroxyarylamine O-acetyltransferase of Salmonella typhimurium. Sequence similarity at the amino acid level suggests a common catalytic mechanism of acetyltransferase for S. typhimurium and higher organisms. J Biol Chem. 1992 Apr 25;267(12):8429-36 PMID:1569093
- ↑ Watanabe M, Igarashi T, Kaminuma T, Sofuni T, Nohmi T. N-hydroxyarylamine O-acetyltransferase of Salmonella typhimurium: proposal for a common catalytic mechanism of arylamine acetyltransferase enzymes. Environ Health Perspect. 1994 Oct;102 Suppl 6(Suppl 6):83-9. PMID:7889864 doi:10.1289/ehp.94102s683
- ↑ Sinclair JC, Sandy J, Delgoda R, Sim E, Noble ME. Structure of arylamine N-acetyltransferase reveals a catalytic triad. Nat Struct Biol. 2000 Jul;7(7):560-4. PMID:10876241 doi:http://dx.doi.org/10.1038/76783
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