1gxp

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(New page: 200px<br /><applet load="1gxp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gxp, resolution 2.5&Aring;" /> '''PHOB EFFECTOR DOMAIN ...)
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[[Image:1gxp.gif|left|200px]]<br /><applet load="1gxp" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gxp, resolution 2.5&Aring;" />
 
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'''PHOB EFFECTOR DOMAIN IN COMPLEX WITH PHO BOX DNA.'''<br />
 
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==Overview==
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==PhoB effector domain in complex with pho box DNA.==
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PhoB is a signal transduction response regulator that activates nearly 40, genes in phosphate depletion conditions in E. coli and closely related, bacteria. The structure of the PhoB effector domain in complex with its, target DNA sequence, or pho box, reveals a novel tandem arrangement in, which several monomers bind head to tail to successive 11-base pair, direct-repeat sequences, coating one face of a smoothly bent double helix., The protein has a winged helix fold in which the DNA recognition elements, comprise helix alpha 3, penetrating the major groove, and a beta hairpin, wing interacting with a compressed minor groove via Arg219, tightly, sandwiched between the DNA sugar backbones. The transactivation loops, protrude laterally in an appropriate orientation to interact with the RNA, polymerase sigma(70) subunit, which triggers transcription initiation.
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<StructureSection load='1gxp' size='340' side='right'caption='[[1gxp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gxp]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The October 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Two-component Systems'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_10 10.2210/rcsb_pdb/mom_2015_10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GXP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gxp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gxp OCA], [https://pdbe.org/1gxp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gxp RCSB], [https://www.ebi.ac.uk/pdbsum/1gxp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gxp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHOB_ECOLI PHOB_ECOLI] This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/1gxp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gxp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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PhoB is a signal transduction response regulator that activates nearly 40 genes in phosphate depletion conditions in E. coli and closely related bacteria. The structure of the PhoB effector domain in complex with its target DNA sequence, or pho box, reveals a novel tandem arrangement in which several monomers bind head to tail to successive 11-base pair direct-repeat sequences, coating one face of a smoothly bent double helix. The protein has a winged helix fold in which the DNA recognition elements comprise helix alpha 3, penetrating the major groove, and a beta hairpin wing interacting with a compressed minor groove via Arg219, tightly sandwiched between the DNA sugar backbones. The transactivation loops protrude laterally in an appropriate orientation to interact with the RNA polymerase sigma(70) subunit, which triggers transcription initiation.
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==About this Structure==
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Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator.,Blanco AG, Sola M, Gomis-Ruth FX, Coll M Structure. 2002 May;10(5):701-13. PMID:12015152<ref>PMID:12015152</ref>
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1GXP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GXP OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Tandem DNA recognition by PhoB, a two-component signal transduction transcriptional activator., Blanco AG, Sola M, Gomis-Ruth FX, Coll M, Structure. 2002 May;10(5):701-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12015152 12015152]
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</div>
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<div class="pdbe-citations 1gxp" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Blanco, A.G.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Coll, M.]]
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[[Category: Two-component Systems]]
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[[Category: Gomis-Ruth, F.X.]]
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[[Category: Blanco AG]]
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[[Category: Sola, M.]]
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[[Category: Coll M]]
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[[Category: activator]]
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[[Category: Gomis-Ruth FX]]
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[[Category: dna binding]]
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[[Category: Sola M]]
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[[Category: helix-winged-helix]]
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[[Category: phosphorylation]]
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[[Category: sensory transduction]]
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[[Category: transcriptional activator]]
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[[Category: two-component signal transduction]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:20:11 2007''
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Current revision

PhoB effector domain in complex with pho box DNA.

PDB ID 1gxp

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