This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1o82

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:57, 9 May 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1o82.jpg|left|200px]]<br /><applet load="1o82" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1o82, resolution 1.46&Aring;" />
 
-
'''X-RAY STRUCTURE OF BACTERIOCIN AS-48 AT PH 4.5. SULPHATE BOUND FORM'''<br />
 
-
==Overview==
+
==X-RAY STRUCTURE OF BACTERIOCIN AS-48 AT PH 4.5. SULPHATE BOUND FORM==
-
The bacteriocin AS-48 is a membrane-interacting peptide, which displays a, broad anti-microbial spectrum against Gram-positive and Gram-negative, bacteria. The NMR structure of AS-48 at pH 3 has been solved. The analysis, of this structure suggests that the mechanism of AS-48 anti-bacterial, activity involves the accumulation of positively charged molecules at the, membrane surface leading to a disruption of the membrane potential. Here, we report the high-resolution crystal structure of AS-48 and sedimentation, equilibrium experiments showing that this bacteriocin is able to adopt, different oligomeric structures according to the physicochemical, environment. The analysis of these structures suggests a mechanism for, molecular function of AS-48 involving a transition from a water-soluble, form to a membrane-bound state upon membrane binding.
+
<StructureSection load='1o82' size='340' side='right'caption='[[1o82]], [[Resolution|resolution]] 1.46&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1o82]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1O82 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.46&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1o82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1o82 OCA], [https://pdbe.org/1o82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1o82 RCSB], [https://www.ebi.ac.uk/pdbsum/1o82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1o82 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q47765_ENTFL Q47765_ENTFL]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The bacteriocin AS-48 is a membrane-interacting peptide, which displays a broad anti-microbial spectrum against Gram-positive and Gram-negative bacteria. The NMR structure of AS-48 at pH 3 has been solved. The analysis of this structure suggests that the mechanism of AS-48 anti-bacterial activity involves the accumulation of positively charged molecules at the membrane surface leading to a disruption of the membrane potential. Here, we report the high-resolution crystal structure of AS-48 and sedimentation equilibrium experiments showing that this bacteriocin is able to adopt different oligomeric structures according to the physicochemical environment. The analysis of these structures suggests a mechanism for molecular function of AS-48 involving a transition from a water-soluble form to a membrane-bound state upon membrane binding.
-
==About this Structure==
+
Structure of bacteriocin AS-48: from soluble state to membrane bound state.,Sanchez-Barrena MJ, Martinez-Ripoll M, Galvez A, Valdivia E, Maqueda M, Cruz V, Albert A J Mol Biol. 2003 Nov 28;334(3):541-9. PMID:14623193<ref>PMID:14623193</ref>
-
1O82 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis] with SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1O82 OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structure of bacteriocin AS-48: from soluble state to membrane bound state., Sanchez-Barrena MJ, Martinez-Ripoll M, Galvez A, Valdivia E, Maqueda M, Cruz V, Albert A, J Mol Biol. 2003 Nov 28;334(3):541-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14623193 14623193]
+
</div>
 +
<div class="pdbe-citations 1o82" style="background-color:#fffaf0;"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Albert, A.]]
+
[[Category: Albert A]]
-
[[Category: Cruz, V.]]
+
[[Category: Cruz V]]
-
[[Category: Galvez, A.]]
+
[[Category: Galvez A]]
-
[[Category: Maqueda, M.]]
+
[[Category: Maqueda M]]
-
[[Category: Martinez-Bueno, M.]]
+
[[Category: Martinez-Bueno M]]
-
[[Category: Martinez-Ripoll, M.]]
+
[[Category: Martinez-Ripoll M]]
-
[[Category: Sanchez-Barrena, M.J.]]
+
[[Category: Sanchez-Barrena MJ]]
-
[[Category: GOL]]
+
-
[[Category: SO4]]
+
-
[[Category: bacteriocin]]
+
-
[[Category: cationic antibacterial peptides]]
+
-
[[Category: cyclic polypeptide]]
+
-
[[Category: membrane permeabilization]]
+
-
[[Category: protein crystallography]]
+
-
[[Category: protein membrane interaction]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 17:00:19 2007''
+

Current revision

X-RAY STRUCTURE OF BACTERIOCIN AS-48 AT PH 4.5. SULPHATE BOUND FORM

PDB ID 1o82

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1o82"
Personal tools