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1qny
From Proteopedia
(Difference between revisions)
(New page: 200px<br /> <applet load="1qny" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qny, resolution 1.8Å" /> '''X-RAY REFINEMENT OF ...) |
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| - | [[Image:1qny.gif|left|200px]]<br /> | ||
| - | <applet load="1qny" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1qny, resolution 1.8Å" /> | ||
| - | '''X-RAY REFINEMENT OF D2O SOAKED CRYSTAL OF CONCANAVALIN A'''<br /> | ||
| - | == | + | ==X-ray refinement of D2O soaked crystal of concanavalin A== |
| - | The correct positions of the deuterium (D) atoms of many of the bound | + | <StructureSection load='1qny' size='340' side='right'caption='[[1qny]], [[Resolution|resolution]] 1.80Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1qny]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QNY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QNY FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qny FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qny OCA], [https://pdbe.org/1qny PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qny RCSB], [https://www.ebi.ac.uk/pdbsum/1qny PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qny ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CONA_CANEN CONA_CANEN] D-mannose specific lectin. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qn/1qny_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qny ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The correct positions of the deuterium (D) atoms of many of the bound waters in the protein concanavalin A are revealed by neutron Laue diffraction. The approach includes cases where these water D atoms show enough mobility to render them invisible even to ultra-high resolution synchrotron-radiation X-ray crystallography. The positions of the bound water H atoms calculated on the basis of chemical and energetic considerations are often incorrect. The D-atom positions for the water molecules in the Mn-, Ca- and sugar-binding sites of concanavalin A are described in detail. | ||
| - | + | Direct determination of the positions of the deuterium atoms of the bound water in -concanavalin A by neutron Laue crystallography.,Habash J, Raftery J, Nuttall R, Price HJ, Wilkinson C, Kalb AJ, Helliwell JR Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):541-50. PMID:10771422<ref>PMID:10771422</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1qny" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Concanavalin 3D structures|Concanavalin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Canavalia ensiformis]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Habash J]] | ||
| + | [[Category: Helliwell JR]] | ||
| + | [[Category: Lehmann MS]] | ||
| + | [[Category: Nuttall R]] | ||
| + | [[Category: Price HJ]] | ||
| + | [[Category: Raftery J]] | ||
| + | [[Category: Wilkinson C]] | ||
Current revision
X-ray refinement of D2O soaked crystal of concanavalin A
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