This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1uty

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:05, 9 May 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1uty.gif|left|200px]]
 
-
<!--
+
==Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)==
-
The line below this paragraph, containing "STRUCTURE_1uty", creates the "Structure Box" on the page.
+
<StructureSection load='1uty' size='340' side='right'caption='[[1uty]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1uty]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bluetongue_virus_8 Bluetongue virus 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UTY FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uty OCA], [https://pdbe.org/1uty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uty RCSB], [https://www.ebi.ac.uk/pdbsum/1uty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uty ProSAT]</span></td></tr>
-
{{STRUCTURE_1uty| PDB=1uty | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''CRYSTAL STRUCTURE OF THE RNA BINDING DOMAIN OF BLUETONGUE VIRUS NON-STRUCTURAL PROTEIN 2(NS2)'''
+
[https://www.uniprot.org/uniprot/VNS2_BTV10 VNS2_BTV10] Single-stranded RNA-binding protein.
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ut/1uty_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uty ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
Bluetongue virus non-structural protein 2 belongs to a class of highly conserved proteins found in orbiviruses of the Reoviridae family. Non-structural protein 2 forms large multimeric complexes and localizes to cytoplasmic inclusions in infected cells. It is able to bind single-stranded RNA non-specifically, and it has been suggested that the protein is involved in the selection and condensation of the Bluetongue virus RNA segments prior to genome encapsidation. We have determined the x-ray structure of the N-terminal domain (sufficient for the RNA binding ability of non-structural protein 2) to 2.4 A resolution using anomalous scattering methods. Crystals of this apparently insoluble domain were obtained by in situ proteolysis of a soluble construct. The asymmetric unit shows two monomers related by non-crystallographic symmetry, with each monomer folded as a beta sandwich with a unique topology. The crystal structure reveals extensive monomer-monomer interactions, which explain the ability of the protein to self-assemble into large homomultimeric complexes. Of the entire surface area of the monomer, one-third is used to create the interfaces of the curved multimeric assembly observed in the x-ray structure. The structure reported here shows how the N-terminal domain would be able to bind single-stranded RNA non-specifically protecting the bound regions in a heterogeneous multimeric but not polymeric complex.
Bluetongue virus non-structural protein 2 belongs to a class of highly conserved proteins found in orbiviruses of the Reoviridae family. Non-structural protein 2 forms large multimeric complexes and localizes to cytoplasmic inclusions in infected cells. It is able to bind single-stranded RNA non-specifically, and it has been suggested that the protein is involved in the selection and condensation of the Bluetongue virus RNA segments prior to genome encapsidation. We have determined the x-ray structure of the N-terminal domain (sufficient for the RNA binding ability of non-structural protein 2) to 2.4 A resolution using anomalous scattering methods. Crystals of this apparently insoluble domain were obtained by in situ proteolysis of a soluble construct. The asymmetric unit shows two monomers related by non-crystallographic symmetry, with each monomer folded as a beta sandwich with a unique topology. The crystal structure reveals extensive monomer-monomer interactions, which explain the ability of the protein to self-assemble into large homomultimeric complexes. Of the entire surface area of the monomer, one-third is used to create the interfaces of the curved multimeric assembly observed in the x-ray structure. The structure reported here shows how the N-terminal domain would be able to bind single-stranded RNA non-specifically protecting the bound regions in a heterogeneous multimeric but not polymeric complex.
-
==About this Structure==
+
Structure and assembly of the RNA binding domain of bluetongue virus non-structural protein 2.,Butan C, Van Der Zandt H, Tucker PA J Biol Chem. 2004 Sep 3;279(36):37613-21. Epub 2004 May 20. PMID:15155766<ref>PMID:15155766</ref>
-
1UTY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus Bluetongue virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTY OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
Structure and assembly of the RNA binding domain of bluetongue virus non-structural protein 2., Butan C, Van Der Zandt H, Tucker PA, J Biol Chem. 2004 Sep 3;279(36):37613-21. Epub 2004 May 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15155766 15155766]
+
</div>
-
[[Category: Bluetongue virus]]
+
<div class="pdbe-citations 1uty" style="background-color:#fffaf0;"></div>
-
[[Category: RNA-directed RNA polymerase]]
+
== References ==
-
[[Category: Single protein]]
+
<references/>
-
[[Category: Butan, C.]]
+
__TOC__
-
[[Category: Tucker, P.]]
+
</StructureSection>
-
[[Category: Zandt, H Van Der.]]
+
[[Category: Bluetongue virus 8]]
-
[[Category: Rna binding protein]]
+
[[Category: Large Structures]]
-
[[Category: Viral protein]]
+
[[Category: Butan C]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:41:08 2008''
+
[[Category: Tucker P]]
 +
[[Category: Van Der zandt H]]

Current revision

Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)

PDB ID 1uty

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1uty"
Personal tools