1w3s
From Proteopedia
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| - | [[Image:1w3s.gif|left|200px]]<br /><applet load="1w3s" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1w3s, resolution 2.40Å" /> | ||
| - | '''THE CRYSTAL STRUCTURE OF RECO FROM DEINOCOCCUS RADIODURANS.'''<br /> | ||
| - | == | + | ==The crystal structure of RecO from Deinococcus radiodurans.== |
| - | The RecFOR pathway has been shown to be essential for DNA repair through | + | <StructureSection load='1w3s' size='340' side='right'caption='[[1w3s]], [[Resolution|resolution]] 2.40Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1w3s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans_R1 Deinococcus radiodurans R1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W3S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W3S FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w3s OCA], [https://pdbe.org/1w3s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w3s RCSB], [https://www.ebi.ac.uk/pdbsum/1w3s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w3s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9RW50_DEIRA Q9RW50_DEIRA] Involved in DNA repair and RecF pathway recombination (By similarity).[HAMAP-Rule:MF_00201][SAAS:SAAS022572_004_002906] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w3/1w3s_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w3s ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The RecFOR pathway has been shown to be essential for DNA repair through the process of homologous recombination in bacteria and, recently, to be important in the recovery of stalled replication forks following UV irradiation. RecO, along with RecR, RecF, RecQ and RecJ, is a principal actor in this fundamental DNA repair pathway. Here we present the three-dimensional structure of a member of the RecO family. The crystal structure of Deinococcus radiodurans RecO (drRecO) reveals possible binding sites for DNA and for the RecO-binding proteins within its three discrete structural regions: an N-terminal oligonucleotide/oligosaccharide-binding domain, a helical bundle and a zinc-finger motif. Furthermore, drRecO was found to form a stable complex with RecR and to bind both single- and double-stranded DNA. Mutational analysis confirmed the existence of multiple DNA-binding sites within the protein. | ||
| - | + | Crystal structure and DNA-binding analysis of RecO from Deinococcus radiodurans.,Leiros I, Timmins J, Hall DR, McSweeney S EMBO J. 2005 Mar 9;24(5):906-18. Epub 2005 Feb 17. PMID:15719017<ref>PMID:15719017</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: Deinococcus radiodurans]] | + | <div class="pdbe-citations 1w3s" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Hall | + | <references/> |
| - | [[Category: Leiros | + | __TOC__ |
| - | [[Category: Leonard | + | </StructureSection> |
| - | [[Category: | + | [[Category: Deinococcus radiodurans R1]] |
| - | [[Category: Timmins | + | [[Category: Large Structures]] |
| - | + | [[Category: Hall DR]] | |
| - | + | [[Category: Leiros I]] | |
| - | + | [[Category: Leonard GA]] | |
| - | + | [[Category: McSweeney SM]] | |
| - | + | [[Category: Timmins J]] | |
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| - | + | ||
| - | + | ||
Current revision
The crystal structure of RecO from Deinococcus radiodurans.
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