1ryk
From Proteopedia
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'''Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;''' | '''Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;''' | ||
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[[Category: Wu, B.]] | [[Category: Wu, B.]] | ||
[[Category: Yee, A.]] | [[Category: Yee, A.]] | ||
| - | [[Category: | + | [[Category: Alpha protein]] |
| - | [[Category: | + | [[Category: Nesg]] |
| - | [[Category: | + | [[Category: Northeast structural genomics consortium]] |
| - | [[Category: | + | [[Category: Ocsp]] |
| - | [[Category: | + | [[Category: Protein structure initiative]] |
| - | [[Category: | + | [[Category: Psi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:04:01 2008'' | |
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Revision as of 05:04, 3 May 2008
Solution NMR Structure Protein yjbJ from Escherichia coli. Northeast Structural Genomics Consortium Target ET93; Ontario Centre for Structural Proteomics target EC0298_1_69;
Overview
The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.
About this Structure
1RYK is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485 Page seeded by OCA on Sat May 3 08:04:01 2008
Categories: Escherichia coli | Single protein | Arrowsmith, C H. | Cort, J R. | Edwards, A M. | Kennedy, M A. | Liao, J. | NESG, Northeast Structural Genomics Consortium. | Pineda-Lucena, A. | Wu, B. | Yee, A. | Alpha protein | Nesg | Northeast structural genomics consortium | Ocsp | Protein structure initiative | Psi | Structural genomic
