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2bl2
From Proteopedia
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| - | [[Image:2bl2.gif|left|200px]]<br /> | ||
| - | <applet load="2bl2" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="2bl2, resolution 2.10Å" /> | ||
| - | '''THE MEMBRANE ROTOR OF THE V-TYPE ATPASE FROM ENTEROCOCCUS HIRAE'''<br /> | ||
| - | == | + | ==The membrane rotor of the V-type ATPase from Enterococcus hirae== |
| - | The membrane rotor ring from the vacuolar-type (V-type) sodium ion-pumping | + | <StructureSection load='2bl2' size='340' side='right'caption='[[2bl2]], [[Resolution|resolution]] 2.10Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2bl2]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_hirae Enterococcus hirae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BL2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BL2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LHG:1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE'>LHG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=UMQ:UNDECYL-MALTOSIDE'>UMQ</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bl2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bl2 OCA], [https://pdbe.org/2bl2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bl2 RCSB], [https://www.ebi.ac.uk/pdbsum/2bl2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bl2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NTPK_ENTHA NTPK_ENTHA] Involved in ATP-driven sodium extrusion. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bl/2bl2_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bl2 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The membrane rotor ring from the vacuolar-type (V-type) sodium ion-pumping adenosine triphosphatase (Na+-ATPase) from Enterococcus hirae consists of 10 NtpK subunits, which are homologs of the 16-kilodalton and 8-kilodalton proteolipids found in other V-ATPases and in F1Fo- or F-ATPases, respectively. Each NtpK subunit has four transmembrane alpha helices, with a sodium ion bound between helices 2 and 4 at a site buried deeply in the membrane that includes the essential residue glutamate-139. This site is probably connected to the membrane surface by two half-channels in subunit NtpI, against which the ring rotates. Symmetry mismatch between the rotor and catalytic domains appears to be an intrinsic feature of both V- and F-ATPases. | ||
| - | + | Structure of the rotor of the V-Type Na+-ATPase from Enterococcus hirae.,Murata T, Yamato I, Kakinuma Y, Leslie AG, Walker JE Science. 2005 Apr 29;308(5722):654-9. Epub 2005 Mar 31. PMID:15802565<ref>PMID:15802565</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 2bl2" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[ATPase 3D structures|ATPase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Enterococcus hirae]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Kakinuma Y]] | ||
| + | [[Category: Leslie AGW]] | ||
| + | [[Category: Murata T]] | ||
| + | [[Category: Walker JE]] | ||
| + | [[Category: Yamato I]] | ||
Current revision
The membrane rotor of the V-type ATPase from Enterococcus hirae
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