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2bsd

From Proteopedia

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Structure of Lactococcal Bacteriophage p2 Receptor Binding Protein

Structural highlights

2bsd is a 3 chain structure with sequence from Escherichia virus P2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RBP_BPLP2 Binds to the host phosphopolysaccharides at the onset of infection. Upon activation by calcium, the receptor binding proteins change their conformation, presenting their binding sites to the host, and a channel opens at the bottom of the baseplate for DNA ejection.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Lactococcus lactis is a Gram-positive bacterium used extensively by the dairy industry for the manufacture of fermented milk products. The double-stranded DNA bacteriophage p2 infects specific L. lactis strains using a receptor-binding protein (RBP) located at the tip of its noncontractile tail. We have solved the crystal structure of phage p2 RBP, a homotrimeric protein composed of three domains: the shoulders, a beta-sandwich attached to the phage; the neck, an interlaced beta-prism; and the receptor-recognition head, a seven-stranded beta-barrel. We used the complex of RBP with a neutralizing llama VHH domain to identify the receptor-binding site. Structural similarity between the recognition-head domain of phage p2 and those of adenoviruses and reoviruses, which invade mammalian cells, suggests that these viruses, despite evolutionary distant targets, lack of sequence similarity and the different chemical nature of their genomes (DNA versus RNA), might have a common ancestral gene.

Lactococcal bacteriophage p2 receptor-binding protein structure suggests a common ancestor gene with bacterial and mammalian viruses.,Spinelli S, Desmyter A, Verrips CT, de Haard HJ, Moineau S, Cambillau C Nat Struct Mol Biol. 2006 Jan;13(1):85-9. Epub 2005 Dec 4. PMID:16327804^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichiere J, van Heel M, Campanacci V, Moineau S, Cambillau C. Structure of lactococcal phage p2 baseplate and its mechanism of activation. Proc Natl Acad Sci U S A. 2010 Mar 29. PMID:20351260
↑ Bebeacua C, Tremblay D, Farenc C, Chapot-Chartier MP, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau C. Structure, adsorption to host, and infection mechanism of virulent lactococcal phage p2. J Virol. 2013 Nov;87(22):12302-12. PMID:24027307 doi:10.1128/JVI.02033-13
↑ Spinelli S, Desmyter A, Verrips CT, de Haard HJ, Moineau S, Cambillau C. Lactococcal bacteriophage p2 receptor-binding protein structure suggests a common ancestor gene with bacterial and mammalian viruses. Nat Struct Mol Biol. 2006 Jan;13(1):85-9. Epub 2005 Dec 4. PMID:16327804 doi:10.1038/nsmb1029

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bsd"

@@ Line 1: / Line 1: @@
-==STRUCTURE OF LACTOCOCCAL BACTERIOPHAGE P2 RECEPTOR BINDING PROTEIN==
-<StructureSection load='2bsd' size='340' side='right' caption='[[2bsd]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+==Structure of Lactococcal Bacteriophage p2 Receptor Binding Protein==
+<StructureSection load='2bsd' size='340' side='right'caption='[[2bsd]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2bsd]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpp2 Bpp2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BSD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BSD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2bsd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_P2 Escherichia virus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BSD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BSD FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bse|2bse]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bsd OCA], [http://pdbe.org/2bsd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bsd RCSB], [http://www.ebi.ac.uk/pdbsum/2bsd PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bsd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bsd OCA], [https://pdbe.org/2bsd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bsd RCSB], [https://www.ebi.ac.uk/pdbsum/2bsd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bsd ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/RBP_BPLP2 RBP_BPLP2] Binds to the host phosphopolysaccharides at the onset of infection. Upon activation by calcium, the receptor binding proteins change their conformation, presenting their binding sites to the host, and a channel opens at the bottom of the baseplate for DNA ejection.<ref>PMID:20351260</ref> <ref>PMID:24027307</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/2bsd_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bs/2bsd_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bpp2]]
+[[Category: Escherichia virus P2]]
-[[Category: Cambillau, C]]
+[[Category: Large Structures]]
-[[Category: Dehaard, H J.W]]
+[[Category: Cambillau C]]
-[[Category: Desmyter, A]]
+[[Category: Dehaard HJW]]
-[[Category: Moineau, S]]
+[[Category: Desmyter A]]
-[[Category: Spinelli, S]]
+[[Category: Moineau S]]
-[[Category: Verrips, C T]]
+[[Category: Spinelli S]]
-[[Category: Lactococcus lacti]]
+[[Category: Verrips CT]]
-[[Category: Llama vhh]]
-[[Category: Phage]]
-[[Category: Receptor]]
-[[Category: Receptor binding protein]]

2bsd

From Proteopedia

Current revision

Structure of Lactococcal Bacteriophage p2 Receptor Binding Protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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