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2chv

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Replication Factor C ADPNP complex

Structural highlights

2chv is a 6 chain structure with sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 4Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RFCS_ARCFU Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We have investigated the communication between subunits in replication factor C (RFC) from Archaeoglobus fulgidus. Mutation of the proposed arginine finger in the small subunits results in a complex that can still bind ATP but has impaired clamp-loading activity, a process that normally only requires binding of nucleotide. The small subunit alone forms a hexameric ring that is six-fold symmetric in the absence of ATP. However, this symmetry is broken when the nucleotide is bound to the complex. A conformational change associated with nucleotide binding may relate to the opening of PCNA rings by RFC during the loading reaction. The structures also reveal the importance of the N-terminal helix of each subunit at the ATP-binding site. Analysis of mutant protein complexes containing subunits lacking this N-terminal helix reveals key distinct regulatory roles during clamp loading that are different for the large and small subunits in the RFC complex.

Communication between subunits within an archaeal clamp-loader complex.,Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB EMBO J. 2006 May 17;25(10):2209-18. Epub 2006 Apr 20. PMID:16628222^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Seybert A, Scott DJ, Scaife S, Singleton MR, Wigley DB. Biochemical characterisation of the clamp/clamp loader proteins from the euryarchaeon Archaeoglobus fulgidus. Nucleic Acids Res. 2002 Oct 15;30(20):4329-38. PMID:12384579
↑ Seybert A, Singleton MR, Cook N, Hall DR, Wigley DB. Communication between subunits within an archaeal clamp-loader complex. EMBO J. 2006 May 17;25(10):2209-18. Epub 2006 Apr 20. PMID:16628222

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2chv"

@@ Line 3: / Line 3: @@
 <StructureSection load='2chv' size='340' side='right'caption='[[2chv]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2chv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CHV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2chv]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CHV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CHV FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2chg|2chg]], [[2chq|2chq]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2chv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2chv OCA], [https://pdbe.org/2chv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2chv RCSB], [https://www.ebi.ac.uk/pdbsum/2chv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2chv ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RFCS_ARCFU RFCS_ARCFU]] Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.<ref>PMID:12384579</ref>
+[https://www.uniprot.org/uniprot/RFCS_ARCFU RFCS_ARCFU] Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity which is further stimulated by PCNA.<ref>PMID:12384579</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 32: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Arcfl]]
+[[Category: Archaeoglobus fulgidus]]
 [[Category: Large Structures]]
-[[Category: Cook, N]]
+[[Category: Cook N]]
-[[Category: Hall, D R]]
+[[Category: Hall DR]]
-[[Category: Seybert, A]]
+[[Category: Seybert A]]
-[[Category: Singleton, M R]]
+[[Category: Singleton MR]]
-[[Category: Wigley, D B]]
+[[Category: Wigley DB]]
-[[Category: Aaa+ atpase]]
-[[Category: Atp-binding]]
-[[Category: Clamp loader]]
-[[Category: Dna binding protein]]
-[[Category: Dna replication]]
-[[Category: Dna-binding protein]]
-[[Category: Nucleotide-binding]]

2chv

From Proteopedia

Current revision

Replication Factor C ADPNP complex

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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