2f77

From Proteopedia

(Difference between revisions)

Current revision

Solution structure of the R55F mutant of M-PMV matrix protein (p10)

Structural highlights

2f77 is a 1 chain structure with sequence from Simian retrovirus 1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GAG_MPMV p10 is the matrix protein. P14 is the nucleocapsid protein. p27 is the capsid protein.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Despite extensive data demonstrating that immature retroviral particle assembly can take place either at the plasma membrane or at a distinct location within the cytoplasm, targeting of viral precursor proteins to either assembly site still remains poorly understood. Biochemical data presented here suggest that Tctex-1, a light chain of the molecular motor dynein, is involved in the intracellular targeting of Mason-Pfizer monkey virus (M-PMV) polyproteins to the cytoplasmic assembly site. Comparison of the three-dimensional structures of M-PMV wild-type matrix protein (wt MA) with a single amino acid mutant (R55F), which redirects assembly from a cytoplasmic site to the plasma membrane, revealed different mutual orientations of their C- and N-terminal domains. This conformational change buries a putative intracellular targeting motif located between both domains in the hydrophobic pocket of the MA molecule, thereby preventing the interaction with cellular transport mechanisms.

D-retrovirus morphogenetic switch driven by the targeting signal accessibility to Tctex-1 of dynein.,Vlach J, Lipov J, Rumlova M, Veverka V, Lang J, Srb P, Knejzlik Z, Pichova I, Hunter E, Hrabal R, Ruml T Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10565-70. Epub 2008 Jul 22. PMID:18647839^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vlach J, Lipov J, Rumlova M, Veverka V, Lang J, Srb P, Knejzlik Z, Pichova I, Hunter E, Hrabal R, Ruml T. D-retrovirus morphogenetic switch driven by the targeting signal accessibility to Tctex-1 of dynein. Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10565-70. Epub 2008 Jul 22. PMID:18647839

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2f77"

@@ Line 1: / Line 1: @@
 ==Solution structure of the R55F mutant of M-PMV matrix protein (p10)==
-<StructureSection load='2f77' size='340' side='right' caption='[[2f77]], [[NMR_Ensembles_of_Models | 18 NMR models]]' scene=''>
+<StructureSection load='2f77' size='340' side='right'caption='[[2f77]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2f77]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Srv-1 Srv-1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F77 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F77 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2f77]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_retrovirus_1 Simian retrovirus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F77 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2f76|2f76]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gag ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11942 SRV-1])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f77 OCA], [https://pdbe.org/2f77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f77 RCSB], [https://www.ebi.ac.uk/pdbsum/2f77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f77 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f77 OCA], [http://pdbe.org/2f77 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f77 RCSB], [http://www.ebi.ac.uk/pdbsum/2f77 PDBsum]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GAG_MPMV GAG_MPMV] p10 is the matrix protein.  P14 is the nucleocapsid protein.  p27 is the capsid protein.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/2f77_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f7/2f77_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 30: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Srv-1]]
+[[Category: Large Structures]]
-[[Category: Hrabal, R]]
+[[Category: Simian retrovirus 1]]
-[[Category: Hunter, E]]
+[[Category: Hrabal R]]
-[[Category: Lang, J]]
+[[Category: Hunter E]]
-[[Category: Lipov, J]]
+[[Category: Lang J]]
-[[Category: Ruml, T]]
+[[Category: Lipov J]]
-[[Category: Rumlova, M]]
+[[Category: Ruml T]]
-[[Category: Srb, P]]
+[[Category: Rumlova M]]
-[[Category: Veverka, V]]
+[[Category: Srb P]]
-[[Category: Vlach, J]]
+[[Category: Veverka V]]
-[[Category: Alpha-helice]]
+[[Category: Vlach J]]
-[[Category: Viral protein]]

2f77

From Proteopedia

Current revision

Solution structure of the R55F mutant of M-PMV matrix protein (p10)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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