2j1v

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[[Image:2j1v.gif|left|200px]]<br />
 
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<applet load="2j1v" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2j1v, resolution 1.45&Aring;" />
 
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'''STRUCTURE OF A STREPTOCOCCUS PNEUMONIAE FUCOSE BINDING MODULE IN COMPLEX WITH THE BLOOD GROUP H-TRISACCHARIDE'''<br />
 
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==Overview==
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==Structure of a Streptococcus pneumoniae fucose binding module in complex with the blood group H-trisaccharide==
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The Streptococcus pneumoniae fucose utilization operon includes a gene, encoding a virulence factor that belongs to family 98 in the glycoside, hydrolase classification. This protein contains a C-terminal triplet of, fucose binding modules that have significant amino acid sequence identity, with the Anguilla anguilla fucolectin. Functional studies of these fucose, binding modules reveal binding to fucosylated oligosaccharides and suggest, the importance of multivalent binding. The high resolution crystal, structures of ligand bound forms of one fucose binding module uncovers the, molecular basis of fucose, ABH blood group antigen, and Lewisy antigen, binding. These studies are extended by fluorescence microscopy to show, specific binding to mouse lung tissue. These modules define a new family, of carbohydrate binding modules now classified as family 47.
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<StructureSection load='2j1v' size='340' side='right'caption='[[2j1v]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2j1v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J1V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J1V FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PRD_900036:H+type+2+antigen,+beta+anomer'>PRD_900036</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j1v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j1v OCA], [https://pdbe.org/2j1v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j1v RCSB], [https://www.ebi.ac.uk/pdbsum/2j1v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j1v ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0H2US34_STRPN A0A0H2US34_STRPN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j1/2j1v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j1v ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Streptococcus pneumoniae fucose utilization operon includes a gene encoding a virulence factor that belongs to family 98 in the glycoside hydrolase classification. This protein contains a C-terminal triplet of fucose binding modules that have significant amino acid sequence identity with the Anguilla anguilla fucolectin. Functional studies of these fucose binding modules reveal binding to fucosylated oligosaccharides and suggest the importance of multivalent binding. The high resolution crystal structures of ligand bound forms of one fucose binding module uncovers the molecular basis of fucose, ABH blood group antigen, and Lewisy antigen binding. These studies are extended by fluorescence microscopy to show specific binding to mouse lung tissue. These modules define a new family of carbohydrate binding modules now classified as family 47.
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==About this Structure==
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Blood group antigen recognition by a Streptococcus pneumoniae virulence factor.,Boraston AB, Wang D, Burke RD J Biol Chem. 2006 Nov 17;281(46):35263-71. Epub 2006 Sep 20. PMID:16987809<ref>PMID:16987809</ref>
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2J1V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with NAG and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J1V OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Blood group antigen recognition by a Streptococcus pneumoniae virulence factor., Boraston AB, Wang D, Burke RD, J Biol Chem. 2006 Nov 17;281(46):35263-71. Epub 2006 Sep 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16987809 16987809]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2j1v" style="background-color:#fffaf0;"></div>
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[[Category: Streptococcus pneumoniae]]
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== References ==
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[[Category: Boraston, A.B.]]
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<references/>
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[[Category: Burke, R.D.]]
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__TOC__
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[[Category: Wang, D.]]
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</StructureSection>
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[[Category: CA]]
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[[Category: Large Structures]]
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[[Category: NAG]]
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[[Category: Streptococcus pneumoniae TIGR4]]
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[[Category: carbohydrate binding protein]]
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[[Category: Boraston AB]]
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[[Category: Burke RD]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 18:23:13 2007''
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[[Category: Wang D]]

Current revision

Structure of a Streptococcus pneumoniae fucose binding module in complex with the blood group H-trisaccharide

PDB ID 2j1v

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