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| | ==NMR structure of Rv2175c== | | ==NMR structure of Rv2175c== |
| - | <StructureSection load='2kfs' size='340' side='right'caption='[[2kfs]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''> | + | <StructureSection load='2kfs' size='340' side='right'caption='[[2kfs]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Myctu Myctu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KFS FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rv2175c ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [https://pdbe.org/2kfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [https://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kfs OCA], [https://pdbe.org/2kfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kfs RCSB], [https://www.ebi.ac.uk/pdbsum/2kfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kfs ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/Y2175_MYCTU Y2175_MYCTU]] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref>
| + | [https://www.uniprot.org/uniprot/Y2175_MYCTU Y2175_MYCTU] Binds DNA at low salt concentrations.<ref>PMID:19457863</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myctu]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Barthe, P]] | + | [[Category: Barthe P]] |
| - | [[Category: Cohen-Gonsaud, M]] | + | [[Category: Cohen-Gonsaud M]] |
| - | [[Category: Molle, V]] | + | [[Category: Molle V]] |
| - | [[Category: Roumestand, C]] | + | [[Category: Roumestand C]] |
| - | [[Category: Dna binding]]
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| - | [[Category: Dna binding protein]]
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| - | [[Category: Dna-binding protein]]
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| - | [[Category: Phosphorylation]]
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| - | [[Category: Whth]]
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| Structural highlights
Function
Y2175_MYCTU Binds DNA at low salt concentrations.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Recent efforts have underlined the role of serine/threonine protein kinases in growth, pathogenesis, and cell wall metabolism in Mycobacterium tuberculosis. Although most kinases have been investigated for their physiological roles, little information is available regarding how serine/threonine protein kinase-dependent phosphorylation regulates the activity of kinase substrates. Herein, we focused on M. tuberculosis Rv2175c, a protein of unknown function, conserved in actinomycetes, and recently identified as a substrate of the PknL kinase. We solved the solution structure of Rv2175c by multidimensional NMR and demonstrated that it possesses an original winged helix-turn-helix motif, indicative of a DNA-binding protein. The DNA-binding activity of Rv2175c was subsequently confirmed by fluorescence anisotropy, as well as in electrophoretic mobility shift assays. Mass spectrometry analyses using a combination of MALDI-TOF and LC-ESI/MS/MS identified Thr(9) as the unique phosphoacceptor. This was further supported by complete loss of PknL-dependent phosphorylation of an Rv2175c_T9A mutant. Importantly, the DNA-binding activity was completely abrogated in a Rv2175c_T9D mutant, designed to mimic constitutive phosphorylation, but not in a mutant lacking the first 13 residues. This implies that the function of the N-terminal extension is to provide a phosphoacceptor (Thr(9)), which, following phosphorylation, negatively regulates the Rv2175c DNA-binding activity. Interestingly, the N-terminal disordered extension, which bears the phosphoacceptor, was found to be restricted to members of the M. tuberculosis complex, thus suggesting the existence of an original mechanism that appears to be unique to the M. tuberculosis complex.
The Mycobacterium tuberculosis Ser/Thr kinase substrate Rv2175c is a DNA-binding protein regulated by phosphorylation.,Cohen-Gonsaud M, Barthe P, Canova MJ, Stagier-Simon C, Kremer L, Roumestand C, Molle V J Biol Chem. 2009 Jul 17;284(29):19290-300. Epub 2009 May 20. PMID:19457863[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cohen-Gonsaud M, Barthe P, Canova MJ, Stagier-Simon C, Kremer L, Roumestand C, Molle V. The Mycobacterium tuberculosis Ser/Thr kinase substrate Rv2175c is a DNA-binding protein regulated by phosphorylation. J Biol Chem. 2009 Jul 17;284(29):19290-300. Epub 2009 May 20. PMID:19457863 doi:10.1074/jbc.M109.019653
- ↑ Cohen-Gonsaud M, Barthe P, Canova MJ, Stagier-Simon C, Kremer L, Roumestand C, Molle V. The Mycobacterium tuberculosis Ser/Thr kinase substrate Rv2175c is a DNA-binding protein regulated by phosphorylation. J Biol Chem. 2009 Jul 17;284(29):19290-300. Epub 2009 May 20. PMID:19457863 doi:10.1074/jbc.M109.019653
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