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| | ==Crystal structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase== | | ==Crystal structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase== |
| - | <StructureSection load='2v78' size='340' side='right' caption='[[2v78]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='2v78' size='340' side='right'caption='[[2v78]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2v78]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/'saccharolobus_solfataricus' 'saccharolobus solfataricus']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V78 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V78 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2v78]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V78 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2var|2var]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructokinase Fructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.4 2.7.1.4] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v78 OCA], [https://pdbe.org/2v78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v78 RCSB], [https://www.ebi.ac.uk/pdbsum/2v78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v78 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v78 OCA], [http://pdbe.org/2v78 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v78 RCSB], [http://www.ebi.ac.uk/pdbsum/2v78 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v78 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KDGK_SULSO KDGK_SULSO]] Involved in the degradation of glucose and galactose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) and 2-keto-3-deoxygalactonate (KDGal) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxy-6-phosphogalactonate (KDPGal), respectively.<ref>PMID:15869466</ref> <ref>PMID:16330030</ref> <ref>PMID:16794308</ref> | + | [https://www.uniprot.org/uniprot/KDGK_SACS2 KDGK_SACS2] Involved in the degradation of glucose and galactose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) and 2-keto-3-deoxygalactonate (KDGal) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxy-6-phosphogalactonate (KDPGal), respectively.<ref>PMID:15869466</ref> <ref>PMID:16330030</ref> <ref>PMID:16794308</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Saccharolobus solfataricus]] | | [[Category: Saccharolobus solfataricus]] |
| - | [[Category: Fructokinase]]
| + | [[Category: Bull SD]] |
| - | [[Category: Bull, S D]] | + | [[Category: Danson MJ]] |
| - | [[Category: Danson, M J]] | + | [[Category: Hough DW]] |
| - | [[Category: Hough, D W]] | + | [[Category: Kerou M]] |
| - | [[Category: Kerou, M]] | + | [[Category: Lamble HJ]] |
| - | [[Category: Lamble, H J]] | + | [[Category: Potter JA]] |
| - | [[Category: Potter, J A]] | + | [[Category: Taylor GL]] |
| - | [[Category: Taylor, G L]] | + | [[Category: Theodossis A]] |
| - | [[Category: Theodossis, A]] | + | |
| - | [[Category: 2-keto-3-deoxygluconate kinase]]
| + | |
| - | [[Category: Kinase]]
| + | |
| - | [[Category: Pfkb family carbohydrate kinase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
KDGK_SACS2 Involved in the degradation of glucose and galactose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) and 2-keto-3-deoxygalactonate (KDGal) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxy-6-phosphogalactonate (KDPGal), respectively.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose and galactose. It has been proposed that a part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus, in which the 2-keto-3-deoxygluconate kinase (KDGK) is promiscuous for both glucose and galactose metabolism. Recombinant S. solfataricus KDGK protein was expressed in Escherichia coli, purified and crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apo S. solfataricus KDGK was solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-keto-3-deoxygluconate (KDGlu) and an ATP analogue was resolved at 2.1 A. The complex suggests that the structural basis for the enzyme's ability to phosphorylate KDGlu and 2-keto-3-deoxygalactonate (KDGal) is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases.
The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase.,Potter JA, Kerou M, Lamble HJ, Bull SD, Hough DW, Danson MJ, Taylor GL Acta Crystallogr D Biol Crystallogr. 2008 Dec;64(Pt 12):1283-7. Epub 2008, Nov 18. PMID:19018105[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ahmed H, Ettema TJ, Tjaden B, Geerling AC, van der Oost J, Siebers B. The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation. Biochem J. 2005 Sep 1;390(Pt 2):529-40. PMID:15869466 doi:http://dx.doi.org/10.1042/BJ20041711
- ↑ Lamble HJ, Theodossis A, Milburn CC, Taylor GL, Bull SD, Hough DW, Danson MJ. Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus. FEBS Lett. 2005 Dec 19;579(30):6865-9. Epub 2005 Dec 1. PMID:16330030 doi:http://dx.doi.org/10.1016/j.febslet.2005.11.028
- ↑ Kim S, Lee SB. Characterization of Sulfolobus solfataricus 2-keto-3-deoxy-D-gluconate kinase in the modified Entner-Doudoroff pathway. Biosci Biotechnol Biochem. 2006 Jun;70(6):1308-16. PMID:16794308 doi:http://dx.doi.org/10.1271/bbb.50566
- ↑ Potter JA, Kerou M, Lamble HJ, Bull SD, Hough DW, Danson MJ, Taylor GL. The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase. Acta Crystallogr D Biol Crystallogr. 2008 Dec;64(Pt 12):1283-7. Epub 2008, Nov 18. PMID:19018105 doi:10.1107/S0907444908036111
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