2vn2

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[[Image:2vn2.png|left|200px]]
 
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{{STRUCTURE_2vn2| PDB=2vn2 | SCENE= }}
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==Crystal structure of the N-terminal domain of DnaD protein from Geobacillus kaustophilus HTA426==
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<StructureSection load='2vn2' size='340' side='right'caption='[[2vn2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2vn2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VN2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vn2 OCA], [https://pdbe.org/2vn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vn2 RCSB], [https://www.ebi.ac.uk/pdbsum/2vn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vn2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5KXY1_GEOKA Q5KXY1_GEOKA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vn/2vn2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vn2 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The DnaD is one of the primosomal proteins that are required for initiation and re-initiation of chromosomal DNA replication in Gram-positive bacteria. The DnaD protein is composed of two major structural domains: an N-terminal oligomerization domain and a C-terminal ssDNA binding domain. Here, we report the crystal structure of the N-terminal domain (aa 1-128) of DnaD (DnaDn) of Geobacillus kaustophilus HTA426 at 2.3A resolution. The structure of DnaDn reveals an extended winged-helix fold, a typical double-stranded DNA binding motif as winged-helix proteins. DnaDn formed tetramers in the crystalline state, but the results of gel filtration chromatography further indicated that this domain of DnaD was a stable dimer in solution. The structural analysis of DnaDn may suggest the binding sites for DNA and DnaB, and an assembly mechanism for Gram-positive bacterial DNA replication primosome.
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===Crystal structure of the N-terminal domain of DnaD protein from Geobacillus kaustophilus HTA426===
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Crystal structure of the N-terminal domain of Geobacillus kaustophilus HTA426 DnaD protein.,Huang CY, Chang YW, Chen WT Biochem Biophys Res Commun. 2008 Oct 17;375(2):220-4. Epub 2008 Aug 12. PMID:18703019<ref>PMID:18703019</ref>
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{{ABSTRACT_PUBMED_18703019}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2vn2" style="background-color:#fffaf0;"></div>
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[[2vn2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Geobacillus_kaustophilus_hta426 Geobacillus kaustophilus hta426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VN2 OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:018703019</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Geobacillus kaustophilus hta426]]
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[[Category: Geobacillus kaustophilus HTA426]]
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[[Category: Chang, Y W.]]
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[[Category: Large Structures]]
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[[Category: Chen, W T.]]
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[[Category: Chang Y-W]]
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[[Category: Hsiao, C D.]]
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[[Category: Chen W-T]]
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[[Category: Huang, C Y.]]
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[[Category: Hsiao C-D]]
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[[Category: Sun, Y J.]]
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[[Category: Huang C-Y]]
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[[Category: Dna replication]]
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[[Category: Sun Y-J]]
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[[Category: Dnad]]
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[[Category: Primosome]]
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[[Category: Replication]]
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Current revision

Crystal structure of the N-terminal domain of DnaD protein from Geobacillus kaustophilus HTA426

PDB ID 2vn2

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