2vn2
From Proteopedia
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| - | {{Seed}} | ||
| - | [[Image:2vn2.png|left|200px]] | ||
| - | < | + | ==Crystal structure of the N-terminal domain of DnaD protein from Geobacillus kaustophilus HTA426== |
| - | + | <StructureSection load='2vn2' size='340' side='right'caption='[[2vn2]], [[Resolution|resolution]] 2.30Å' scene=''> | |
| - | You may | + | == Structural highlights == |
| - | + | <table><tr><td colspan='2'>[[2vn2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VN2 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |
| - | - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vn2 OCA], [https://pdbe.org/2vn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vn2 RCSB], [https://www.ebi.ac.uk/pdbsum/2vn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vn2 ProSAT]</span></td></tr> | |
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q5KXY1_GEOKA Q5KXY1_GEOKA] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vn/2vn2_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vn2 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The DnaD is one of the primosomal proteins that are required for initiation and re-initiation of chromosomal DNA replication in Gram-positive bacteria. The DnaD protein is composed of two major structural domains: an N-terminal oligomerization domain and a C-terminal ssDNA binding domain. Here, we report the crystal structure of the N-terminal domain (aa 1-128) of DnaD (DnaDn) of Geobacillus kaustophilus HTA426 at 2.3A resolution. The structure of DnaDn reveals an extended winged-helix fold, a typical double-stranded DNA binding motif as winged-helix proteins. DnaDn formed tetramers in the crystalline state, but the results of gel filtration chromatography further indicated that this domain of DnaD was a stable dimer in solution. The structural analysis of DnaDn may suggest the binding sites for DNA and DnaB, and an assembly mechanism for Gram-positive bacterial DNA replication primosome. | ||
| - | + | Crystal structure of the N-terminal domain of Geobacillus kaustophilus HTA426 DnaD protein.,Huang CY, Chang YW, Chen WT Biochem Biophys Res Commun. 2008 Oct 17;375(2):220-4. Epub 2008 Aug 12. PMID:18703019<ref>PMID:18703019</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | == | + | </div> |
| - | + | <div class="pdbe-citations 2vn2" style="background-color:#fffaf0;"></div> | |
| - | [[Category: | + | == References == |
| - | [[Category: Chang | + | <references/> |
| - | [[Category: Chen | + | __TOC__ |
| - | [[Category: Hsiao | + | </StructureSection> |
| - | [[Category: Huang | + | [[Category: Geobacillus kaustophilus HTA426]] |
| - | [[Category: Sun | + | [[Category: Large Structures]] |
| - | + | [[Category: Chang Y-W]] | |
| - | + | [[Category: Chen W-T]] | |
| - | + | [[Category: Hsiao C-D]] | |
| - | + | [[Category: Huang C-Y]] | |
| - | + | [[Category: Sun Y-J]] | |
| - | + | ||
Current revision
Crystal structure of the N-terminal domain of DnaD protein from Geobacillus kaustophilus HTA426
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