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| | ==Structure of SmeT, the repressor of the Stenotrophomonas maltophilia multidrug efflux pump SmeDEF.== | | ==Structure of SmeT, the repressor of the Stenotrophomonas maltophilia multidrug efflux pump SmeDEF.== |
| - | <StructureSection load='2w53' size='340' side='right' caption='[[2w53]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='2w53' size='340' side='right'caption='[[2w53]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2w53]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"pseudomonas_maltophilia"_hugh_and_ryschenkow_1961 "pseudomonas maltophilia" hugh and ryschenkow 1961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W53 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W53 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2w53]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stenotrophomonas_maltophilia Stenotrophomonas maltophilia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W53 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w53 OCA], [http://pdbe.org/2w53 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2w53 RCSB], [http://www.ebi.ac.uk/pdbsum/2w53 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2w53 ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w53 OCA], [https://pdbe.org/2w53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w53 RCSB], [https://www.ebi.ac.uk/pdbsum/2w53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w53 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8KLP4_STEMA Q8KLP4_STEMA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pseudomonas maltophilia hugh and ryschenkow 1961]] | + | [[Category: Large Structures]] |
| - | [[Category: Hernandez, A]] | + | [[Category: Stenotrophomonas maltophilia]] |
| - | [[Category: Martinez, J L]] | + | [[Category: Hernandez A]] |
| - | [[Category: Mate, M J]]
| + | [[Category: Martinez JL]] |
| - | [[Category: Romero, A]]
| + | [[Category: Mate MJ]] |
| - | [[Category: Antibiotic resistance]] | + | [[Category: Romero A]] |
| - | [[Category: Dna binding]] | + | |
| - | [[Category: Dna-binding]] | + | |
| - | [[Category: Multi-drug efflux pump]]
| + | |
| - | [[Category: Tetr family]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcription regulation]]
| + | |
| - | [[Category: Transcriptional repressor]]
| + | |
| Structural highlights
Function
Q8KLP4_STEMA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Stenotrophomonas maltophilia is an opportunistic pathogen characterized for its intrinsic low susceptibility to several antibiotics. Part of this low susceptibility relies on the expression of chromosomally encoded multidrug efflux pumps, with SmeDEF being the most relevant antibiotic resistance efflux pump so far studied in this bacterial species. Expression of smeDEF is down-regulated by the SmeT repressor, encoded upstream smeDEF, in its complementary DNA strand. In the present article we present the crystal structure of SmeT and analyze its interactions with its cognate operator. Like other members of the TetR family of transcriptional repressors, SmeT behaves as a dimer and presents some common structural features with other TetR proteins like TtgR, QacR, and TetR. Differing from other TetR proteins for which the structure is available, SmeT turned out to have two extensions at the N and C termini that might be relevant for its function. Besides, SmeT presents the smallest binding pocket so far described in the TetR family of transcriptional repressors, which may correlate with a specific type and range of effectors. In vitro studies revealed that SmeT binds to a 28-bp pseudopalindromic region, forming two complexes. This operator region was found to overlap the promoters of smeT and smeDEF. This finding is consistent with a role for SmeT simultaneously down-regulating smeT and smeDEF transcription, likely by steric hindrance on RNA polymerase binding to DNA.
Structural and functional analysis of SmeT, the repressor of the Stenotrophomonas maltophilia multidrug efflux pump SmeDEF.,Hernandez A, Mate MJ, Sanchez-Diaz PC, Romero A, Rojo F, Martinez JL J Biol Chem. 2009 May 22;284(21):14428-38. Epub 2009 Mar 26. PMID:19324881[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hernandez A, Mate MJ, Sanchez-Diaz PC, Romero A, Rojo F, Martinez JL. Structural and functional analysis of SmeT, the repressor of the Stenotrophomonas maltophilia multidrug efflux pump SmeDEF. J Biol Chem. 2009 May 22;284(21):14428-38. Epub 2009 Mar 26. PMID:19324881 doi:10.1074/jbc.M809221200
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