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3zuw

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Photosynthetic Reaction Centre Mutant with TYR L128 replaced with HIS

Structural highlights

3zuw is a 3 chain structure with sequence from Cereibacter sphaeroides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.31Å
Ligands:	, , , , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RCEH_CERSP The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.

Publication Abstract from PubMed

The kinetics of recombination of the P(+)H(A)(-) radical pair were compared in wild-type reaction centers from Rhodobacter sphaeroides and in seven mutants in which the free energy gap, DeltaG, between the charge separated states P(+)B(A)(-) and P(+)H(A)(-) was either increased or decreased. Five of the mutant RCs had been described previously, and X-ray crystal structures of two newly constructed complexes were determined by X-ray crystallography. The charge recombination reaction was accelerated in all mutants with a smaller DeltaG than in the wild-type, and was slowed in a mutant having a larger DeltaG. The free energy difference between the state P(+)H(A)(-) and the PH(A) ground state was unaffected by most of these mutations. These observations were consistent with a model in which the P(+)H(A)(-) --> PH(A) charge recombination is thermally activated and occurs via the intermediate state P(+)B(A)(-), with a mean rate related to the size of the DeltaG between the states P(+)B(A)(-) and P(+)H(A)(-) and not the DeltaG between P(+)H(A)(-) and the ground state. A more detailed analysis of charge recombination in the mutants showed that the kinetics of the reaction were multiexponential, and characterized by approximately 0.5, approximately 1-3, and 7-17 ns lifetimes, similar to those measured for wild-type reaction centers. The exact lifetimes and relative amplitudes of the three components were strongly modulated by the mutations. Two models were considered in order to explain the observed multiexponentiality and modulation, involving heterogeneity or relaxation of P(+)H(A)(-) states, with the latter model giving a better fit to the experimental results.

Mechanism of Recombination of the P(+)H(A)(-) Radical Pair in Mutant Rhodobacter sphaeroides Reaction Centers with Modified Free Energy Gaps Between P(+)B(A)(-) and P(+)H(A)(-).,Gibasiewicz K, Pajzderska M, Potter JA, Fyfe PK, Dobek A, Brettel K, Jones MR J Phys Chem B. 2011 Nov 10;115(44):13037-50. Epub 2011 Oct 18. PMID:21970763^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gibasiewicz K, Pajzderska M, Potter JA, Fyfe PK, Dobek A, Brettel K, Jones MR. Mechanism of Recombination of the P(+)H(A)(-) Radical Pair in Mutant Rhodobacter sphaeroides Reaction Centers with Modified Free Energy Gaps Between P(+)B(A)(-) and P(+)H(A)(-). J Phys Chem B. 2011 Nov 10;115(44):13037-50. Epub 2011 Oct 18. PMID:21970763 doi:10.1021/jp206462g

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zuw"

@@ Line 1: / Line 1: @@
-==PHOTOSYNTHETIC REACTION CENTRE MUTANT WITH TYR L128 REPLACED WITH HIS==
+==Photosynthetic Reaction Centre Mutant with TYR L128 replaced with HIS==
-<StructureSection load='3zuw' size='340' side='right' caption='[[3zuw]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
+<StructureSection load='3zuw' size='340' side='right'caption='[[3zuw]], [[Resolution|resolution]] 2.31&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zuw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"rhodococcus_capsulatus"_molisch_1907 "rhodococcus capsulatus" molisch 1907]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZUW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zuw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZUW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZUW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.31&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ux4|2ux4]], [[1dv6|1dv6]], [[1pcr|1pcr]], [[1dv3|1dv3]], [[1jgw|1jgw]], [[1qov|1qov]], [[2jj0|2jj0]], [[1m3x|1m3x]], [[1rzz|1rzz]], [[1ds8|1ds8]], [[1aij|1aij]], [[1pss|1pss]], [[1fnp|1fnp]], [[1rqk|1rqk]], [[1kby|1kby]], [[2gmr|2gmr]], [[1rvj|1rvj]], [[2uxk|2uxk]], [[2uww|2uww]], [[1l9j|1l9j]], [[1yst|1yst]], [[3zum|3zum]], [[1jh0|1jh0]], [[1k6l|1k6l]], [[2uxl|2uxl]], [[1umx|1umx]], [[1mps|1mps]], [[1pst|1pst]], [[1rg5|1rg5]], [[1f6n|1f6n]], [[2ux5|2ux5]], [[1k6n|1k6n]], [[1ry5|1ry5]], [[1e6d|1e6d]], [[2bnp|2bnp]], [[1fnq|1fnq]], [[2j8d|2j8d]], [[1rzh|1rzh]], [[2uws|2uws]], [[2boz|2boz]], [[1ogv|1ogv]], [[2uwt|2uwt]], [[1jgx|1jgx]], [[4rcr|4rcr]], [[1jgz|1jgz]], [[2uwu|2uwu]], [[2jiy|2jiy]], [[2uwv|2uwv]], [[1rgn|1rgn]], [[2uxj|2uxj]], [[1jgy|1jgy]], [[2uxm|2uxm]], [[2rcr|2rcr]], [[2j8c|2j8c]], [[2ux3|2ux3]], [[1s00|1s00]], [[2bns|2bns]], [[1l9b|1l9b]], [[1e14|1e14]], [[1aig|1aig]], [[1z9k|1z9k]], [[1z9j|1z9j]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zuw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zuw OCA], [http://pdbe.org/3zuw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zuw RCSB], [http://www.ebi.ac.uk/pdbsum/3zuw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zuw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zuw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zuw OCA], [https://pdbe.org/3zuw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zuw RCSB], [https://www.ebi.ac.uk/pdbsum/3zuw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zuw ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RCEH_RHOSH RCEH_RHOSH]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/RCEM_RHOSH RCEM_RHOSH]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis. [[http://www.uniprot.org/uniprot/RCEL_RHOSH RCEL_RHOSH]] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
+[https://www.uniprot.org/uniprot/RCEH_CERSP RCEH_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Rhodococcus capsulatus molisch 1907]]
+[[Category: Cereibacter sphaeroides]]
-[[Category: Brettel, K]]
+[[Category: Large Structures]]
-[[Category: Dobek, A]]
+[[Category: Brettel K]]
-[[Category: Fyfe, P K]]
+[[Category: Dobek A]]
-[[Category: Gibasiewicz, K]]
+[[Category: Fyfe PK]]
-[[Category: Jones, M R]]
+[[Category: Gibasiewicz K]]
-[[Category: Pajzderska, M]]
+[[Category: Jones MR]]
-[[Category: Potter, J A]]
+[[Category: Pajzderska M]]
-[[Category: Charge recombination]]
+[[Category: Potter JA]]
-[[Category: Electron transfer]]
-[[Category: Photosynthesis]]
-[[Category: Primary charge separation]]
-[[Category: Transient absorption spectroscopy]]

3zuw

From Proteopedia

Current revision

Photosynthetic Reaction Centre Mutant with TYR L128 replaced with HIS

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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