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| | ==Structure of hDcp1a in complex with proline rich sequence of PNRC2== | | ==Structure of hDcp1a in complex with proline rich sequence of PNRC2== |
| - | <StructureSection load='4b6h' size='340' side='right' caption='[[4b6h]], [[Resolution|resolution]] 2.60Å' scene=''> | + | <StructureSection load='4b6h' size='340' side='right'caption='[[4b6h]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4b6h]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B6H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4b6h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4B6H FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b6h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b6h RCSB], [http://www.ebi.ac.uk/pdbsum/4b6h PDBsum]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4b6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b6h OCA], [https://pdbe.org/4b6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4b6h RCSB], [https://www.ebi.ac.uk/pdbsum/4b6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4b6h ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DCP1A_HUMAN DCP1A_HUMAN]] Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.<ref>PMID:11836524</ref> <ref>PMID:12417715</ref> | + | [https://www.uniprot.org/uniprot/DCP1A_HUMAN DCP1A_HUMAN] Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.<ref>PMID:11836524</ref> <ref>PMID:12417715</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 4b6h" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| - | [[Category: Lai, T]] | + | [[Category: Large Structures]] |
| - | [[Category: Song, H]] | + | [[Category: Lai T]] |
| - | [[Category: Decapping]] | + | [[Category: Song H]] |
| - | [[Category: Hydrolase-peptide complex]]
| + | |
| Structural highlights
Function
DCP1A_HUMAN Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.[1] [2]
Publication Abstract from PubMed
Nonsense-mediated mRNA decay (NMD) is an important mRNA surveillance system, and human PNRC2 protein mediates the link between mRNA surveillance and decapping. However, the mechanism by which PNRC2 interacts with the mRNA surveillance machinery and stimulates NMD is unknown. Here, we present the crystal structure of Dcp1a in complex with PNRC2. The proline-rich region of PNRC2 is bound to the EVH1 domain of Dcp1a, while its NR-box mediates the interaction with the hyperphosphorylated Upf1. The mode of PNRC2 interaction with Dcp1a is distinct from those observed in other EVH1/proline-rich ligands interactions. Disruption of the interaction of PNRC2 with Dcp1a abolishes its P-body localization and ability to promote mRNA degradation when tethered to mRNAs. PNRC2 acts in synergy with Dcp1a to stimulate the decapping activity of Dcp2 by bridging the interaction between Dcp1a and Dcp2, suggesting that PNRC2 is a decapping coactivator in addition to its adaptor role in NMD.
Structural basis of the PNRC2-mediated link between mrna surveillance and decapping.,Lai T, Cho H, Liu Z, Bowler MW, Piao S, Parker R, Kim YK, Song H Structure. 2012 Dec 5;20(12):2025-37. doi: 10.1016/j.str.2012.09.009. Epub 2012, Oct 18. PMID:23085078[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bai RY, Koester C, Ouyang T, Hahn SA, Hammerschmidt M, Peschel C, Duyster J. SMIF, a Smad4-interacting protein that functions as a co-activator in TGFbeta signalling. Nat Cell Biol. 2002 Mar;4(3):181-90. PMID:11836524 doi:http://dx.doi.org/10.1038/ncb753
- ↑ Lykke-Andersen J. Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay. Mol Cell Biol. 2002 Dec;22(23):8114-21. PMID:12417715
- ↑ Lai T, Cho H, Liu Z, Bowler MW, Piao S, Parker R, Kim YK, Song H. Structural basis of the PNRC2-mediated link between mrna surveillance and decapping. Structure. 2012 Dec 5;20(12):2025-37. doi: 10.1016/j.str.2012.09.009. Epub 2012, Oct 18. PMID:23085078 doi:http://dx.doi.org/10.1016/j.str.2012.09.009
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