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4u1c

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Crystal structure of the eIF3a/eIF3c PCI-domain heterodimer

Structural highlights

4u1c is a 2 chain structure with sequence from Saccharomyces cerevisiae S288C. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.5Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

EIF3C_YEAST Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.^[1]

Publication Abstract from PubMed

Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, six-subunit Saccharomyces cerevisiae eIF3 core. These structures, together with electron microscopy reconstructions, cross-linking coupled to mass spectrometry, and integrative structure modeling, allowed us to position and orient all eIF3 components on the 40SeIF1 complex, revealing an extended, modular arrangement of eIF3 subunits. Yeast eIF3 engages 40S in a clamp-like manner, fully encircling 40S to position key initiation factors on opposite ends of the mRNA channel, providing a platform for the recruitment, assembly, and regulation of the translation initiation machinery. The structures of eIF3 components reported here also have implications for understanding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and the hepatitis C internal ribosomal entry site RNA.

Molecular Architecture of the 40SeIF1eIF3 Translation Initiation Complex.,Erzberger JP, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett CH, Cimermancic P, Boehringer D, Sali A, Aebersold R, Ban N Cell. 2014 Aug 28;158(5):1123-35. doi: 10.1016/j.cell.2014.07.044. PMID:25171412^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Valasek L, Mathew AA, Shin BS, Nielsen KH, Szamecz B, Hinnebusch AG. The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo. Genes Dev. 2003 Mar 15;17(6):786-99. PMID:12651896 doi:http://dx.doi.org/10.1101/gad.1065403
↑ Erzberger JP, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett CH, Cimermancic P, Boehringer D, Sali A, Aebersold R, Ban N. Molecular Architecture of the 40SeIF1eIF3 Translation Initiation Complex. Cell. 2014 Aug 28;158(5):1123-35. doi: 10.1016/j.cell.2014.07.044. PMID:25171412 doi:http://dx.doi.org/10.1016/j.cell.2014.07.044

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4u1c"

Categories: Large Structures | Saccharomyces cerevisiae S288C | Ban N | Erzberger JP | Schaefer T

@@ Line 1: / Line 1: @@
 ==Crystal structure of the eIF3a/eIF3c PCI-domain heterodimer==
-<StructureSection load='4u1c' size='340' side='right' caption='[[4u1c]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
+<StructureSection load='4u1c' size='340' side='right'caption='[[4u1c]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4u1c]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U1C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4U1C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4u1c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U1C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U1C FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4u1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u1c OCA], [http://pdbe.org/4u1c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4u1c RCSB], [http://www.ebi.ac.uk/pdbsum/4u1c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4u1c ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u1c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u1c OCA], [https://pdbe.org/4u1c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u1c RCSB], [https://www.ebi.ac.uk/pdbsum/4u1c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u1c ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/EIF3C_YEAST EIF3C_YEAST]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.<ref>PMID:12651896</ref>  [[http://www.uniprot.org/uniprot/EIF3A_YEAST EIF3A_YEAST]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.<ref>PMID:9694884</ref> <ref>PMID:11387228</ref> <ref>PMID:12651896</ref> <ref>PMID:18765792</ref>
+[https://www.uniprot.org/uniprot/EIF3C_YEAST EIF3C_YEAST] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome.<ref>PMID:12651896</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 20: @@
 ==See Also==
-*[[Eukaryotic initiation factor|Eukaryotic initiation factor]]
+*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ban, N]]
+[[Category: Large Structures]]
-[[Category: Erzberger, J P]]
+[[Category: Saccharomyces cerevisiae S288C]]
-[[Category: Schaefer, T]]
+[[Category: Ban N]]
-[[Category: Eif3 complex]]
+[[Category: Erzberger JP]]
-[[Category: Pci-domain]]
+[[Category: Schaefer T]]
-[[Category: Translation]]
-[[Category: Translation initiation]]

4u1c

From Proteopedia

Current revision

Crystal structure of the eIF3a/eIF3c PCI-domain heterodimer

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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