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5c6g

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Structural Insights into the Scc2-Scc4 Cohesin Loader

Structural highlights

5c6g is a 4 chain structure with sequence from Eremothecium gossypii ATCC 10895. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.6Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q74ZR5_ASHGO

Publication Abstract from PubMed

The remarkable accuracy of eukaryotic cell division is partly maintained by the cohesin complex acting as a molecular glue to prevent premature sister chromatid separation. The loading of cohesin onto chromosomes is catalyzed by the Scc2-Scc4 loader complex. Here, we report the crystal structure of Scc4 bound to the N terminus of Scc2 and show that Scc4 is a tetratricopeptide repeat (TPR) superhelix. The Scc2 N terminus adopts an extended conformation and is entrapped by the core of the Scc4 superhelix. Electron microscopy (EM) analysis reveals that the Scc2-Scc4 loader complex comprises three domains: a head, body, and hook. Deletion studies unambiguously assign the Scc2N-Scc4 as the globular head domain, whereas in vitro cohesin loading assays show that the central body and the hook domains are sufficient to catalyze cohesin loading onto circular DNA, but not chromatinized DNA in vivo, suggesting a possible role for Scc4 as a chromatin adaptor.

Structural Studies Reveal the Functional Modularity of the Scc2-Scc4 Cohesin Loader.,Chao WC, Murayama Y, Munoz S, Costa A, Uhlmann F, Singleton MR Cell Rep. 2015 Jul 22. pii: S2211-1247(15)00709-3. doi:, 10.1016/j.celrep.2015.06.071. PMID:26212329^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chao WC, Murayama Y, Munoz S, Costa A, Uhlmann F, Singleton MR. Structural Studies Reveal the Functional Modularity of the Scc2-Scc4 Cohesin Loader. Cell Rep. 2015 Jul 22. pii: S2211-1247(15)00709-3. doi:, 10.1016/j.celrep.2015.06.071. PMID:26212329 doi:http://dx.doi.org/10.1016/j.celrep.2015.06.071

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5c6g"

Categories: Eremothecium gossypii ATCC 10895 | Large Structures | Chao WCH | Singleton MR

@@ Line 3: / Line 3: @@
 <StructureSection load='5c6g' size='340' side='right'caption='[[5c6g]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5c6g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Ashgo Ashgo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C6G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5c6g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Eremothecium_gossypii_ATCC_10895 Eremothecium gossypii ATCC 10895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C6G FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AGOS_AGR133C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284811 ASHGO]), SCC2, AGL133W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284811 ASHGO])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6g OCA], [http://pdbe.org/5c6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c6g RCSB], [http://www.ebi.ac.uk/pdbsum/5c6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6g ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6g OCA], [https://pdbe.org/5c6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c6g RCSB], [https://www.ebi.ac.uk/pdbsum/5c6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6g ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SCC2_ASHGO SCC2_ASHGO]] Plays a structural role in chromatin. Probably involved in sister chromatid cohesion, possibly by interacting with the cohesin complex (By similarity).
+[https://www.uniprot.org/uniprot/Q74ZR5_ASHGO Q74ZR5_ASHGO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Ashgo]]
+[[Category: Eremothecium gossypii ATCC 10895]]
 [[Category: Large Structures]]
-[[Category: Chao, W C.H]]
+[[Category: Chao WCH]]
-[[Category: Singleton, M R]]
+[[Category: Singleton MR]]
-[[Category: Cell cycle]]
-[[Category: Cohesin loader]]
-[[Category: Tpr repeat]]

5c6g

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Current revision

Structural Insights into the Scc2-Scc4 Cohesin Loader

Structural highlights

Function

Publication Abstract from PubMed

References

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