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1s18

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[[Image:1s18.gif|left|200px]]
[[Image:1s18.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1s18 |SIZE=350|CAPTION= <scene name='initialview01'>1s18</scene>, resolution 1.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1s18", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Apyrase Apyrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.5 3.6.1.5] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= SHAPY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1s18| PDB=1s18 | SCENE= }}
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|RELATEDENTRY=[[1s1d|1S1D]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s18 OCA], [http://www.ebi.ac.uk/pdbsum/1s18 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s18 RCSB]</span>
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}}
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'''Structure and protein design of human apyrase'''
'''Structure and protein design of human apyrase'''
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[[Category: Lu, M.]]
[[Category: Lu, M.]]
[[Category: Smith, T M.]]
[[Category: Smith, T M.]]
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[[Category: adpase,five-blade beta propeller,calcium-binding protein]]
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[[Category: Adpase,five-blade beta propeller,calcium-binding protein]]
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[[Category: nucleotide-binding motif]]
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[[Category: Nucleotide-binding motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:10:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:35:53 2008''
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Revision as of 05:10, 3 May 2008

Image:1s18.gif

Template:STRUCTURE 1s18

Structure and protein design of human apyrase


Overview

Hematophagous arthropods secrete a salivary apyrase that inhibits platelet activation by catabolizing ADP released from damaged tissues and blood cells. We report the X-ray crystal structures of a human enzyme of the soluble apyrase family in its apo state and bound to a substrate analog. The structures reveal a nucleotide binding domain comprising a five-blade beta propeller, binding determinants of the substrate and the active site, and an unusual calcium binding site with a potential regulatory function. Using a comparative structural biology approach, we were able to redesign the human apyrase so as to enhance its ADPase activity by more than 100-fold. The engineered enzyme is a potent inhibitor of platelet aggregation and may serve as the basis for the development of a new class of antithrombotic agents.

About this Structure

1S18 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure and protein design of a human platelet function inhibitor., Dai J, Liu J, Deng Y, Smith TM, Lu M, Cell. 2004 Mar 5;116(5):649-59. PMID:15006348 Page seeded by OCA on Sat May 3 08:10:01 2008

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