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6f8h

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==antitoxin GraA==
==antitoxin GraA==
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<StructureSection load='6f8h' size='340' side='right' caption='[[6f8h]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='6f8h' size='340' side='right'caption='[[6f8h]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6f8h]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6F8H FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6f8h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6F8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6F8H FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6f8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f8h OCA], [http://pdbe.org/6f8h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6f8h RCSB], [http://www.ebi.ac.uk/pdbsum/6f8h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6f8h ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.002&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6f8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6f8h OCA], [https://pdbe.org/6f8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6f8h RCSB], [https://www.ebi.ac.uk/pdbsum/6f8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6f8h ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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The graTA operon from Pseudomonas putida encodes a toxin-antitoxin module with an unusually moderate toxin. Here, the production, SAXS analysis and crystallization of the antitoxin GraA, the GraTA complex and the complex of GraA with a 33 bp operator fragment are reported. GraA forms a homodimer in solution and crystallizes in space group P21, with unit-cell parameters a = 66.9, b = 48.9, c = 62.7 A, beta = 92.6 degrees . The crystals are likely to contain two GraA dimers in the asymmetric unit and diffract to 1.9 A resolution. The GraTA complex forms a heterotetramer in solution. Crystals of the GraTA complex diffracted to 2.2 A resolution and are most likely to contain a single heterotetrameric GraTA complex in the asymmetric unit. They belong to space group P41 or P43, with unit-cell parameters a = b = 56.0, c = 128.2 A. The GraA-operator complex consists of a 33 bp operator region that binds two GraA dimers. It crystallizes in space group P31 or P32, with unit-cell parameters a = b = 105.6, c = 149.9 A. These crystals diffract to 3.8 A resolution.
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Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraT2A2 complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity.
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Production, biophysical characterization and crystallization of Pseudomonas putida GraA and its complexes with GraT and the graTA operator.,Talavera A, Tamman H, Ainelo A, Hadaei S, Garcia-Pino A, Horak R, Konijnenberg A, Loris R Acta Crystallogr F Struct Biol Commun. 2017 Aug 1;73(Pt 8):455-462. doi:, 10.1107/S2053230X17009438. Epub 2017 Jul 26. PMID:28777088<ref>PMID:28777088</ref>
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A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.,Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadzi S, Sobott F, Garcia-Pino A, Horak R, Loris R Nat Commun. 2019 Feb 27;10(1):972. doi: 10.1038/s41467-019-08865-z. PMID:30814507<ref>PMID:30814507</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Loris, R]]
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[[Category: Large Structures]]
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[[Category: Talavera, A]]
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[[Category: Pseudomonas putida]]
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[[Category: Antitoxin]]
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[[Category: Loris R]]
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[[Category: Graa]]
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[[Category: Talavera A]]
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[[Category: Higa]]
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antitoxin GraA

PDB ID 6f8h

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