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1upn

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[[Image:1upn.gif|left|200px]]<br />
 
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<applet load="1upn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1upn" />
 
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'''COMPLEX OF ECHOVIRUS TYPE 12 WITH DOMAINS 3 AND 4 OF ITS RECEPTOR DECAY ACCELERATING FACTOR (CD55) BY CRYO ELECTRON MICROSCOPY AT 16 A'''<br />
 
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==Overview==
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==COMPLEX OF ECHOVIRUS TYPE 12 WITH DOMAINS 3 AND 4 OF ITS RECEPTOR DECAY ACCELERATING FACTOR (CD55) BY CRYO ELECTRON MICROSCOPY AT 16 A==
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Echovirus type 12 (EV12), an Enterovirus of the Picornaviridae family, uses the complement regulator decay-accelerating factor (DAF, CD55) as a, cellular receptor. We have calculated a three-dimensional reconstruction, of EV12 bound to a fragment of DAF consisting of short consensus repeat, domains 3 and 4 from cryo-negative stain electron microscopy data (EMD, code 1057). This shows that, as for an earlier reconstruction of the, related echovirus type 7 bound to DAF, attachment is not within the viral, canyon but occurs close to the 2-fold symmetry axes. Despite this general, similarity our reconstruction reveals a receptor interaction that is quite, different from that observed for EV7. Fitting of the crystallographic, co-ordinates for DAF(34) and EV11 into the reconstruction shows a ... [[http://ispc.weizmann.ac.il/pmbin/getpm?14634014 (full description)]]
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<SX load='1upn' size='340' side='right' viewer='molstar' caption='[[1upn]], [[Resolution|resolution]] 16.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1upn]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Echovirus_E11 Echovirus E11] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UPN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 16&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1upn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1upn OCA], [https://pdbe.org/1upn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1upn RCSB], [https://www.ebi.ac.uk/pdbsum/1upn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1upn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DAF_HUMAN DAF_HUMAN] This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.<ref>PMID:7525274</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/up/1upn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1upn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Echovirus type 12 (EV12), an Enterovirus of the Picornaviridae family, uses the complement regulator decay-accelerating factor (DAF, CD55) as a cellular receptor. We have calculated a three-dimensional reconstruction of EV12 bound to a fragment of DAF consisting of short consensus repeat domains 3 and 4 from cryo-negative stain electron microscopy data (EMD code 1057). This shows that, as for an earlier reconstruction of the related echovirus type 7 bound to DAF, attachment is not within the viral canyon but occurs close to the 2-fold symmetry axes. Despite this general similarity our reconstruction reveals a receptor interaction that is quite different from that observed for EV7. Fitting of the crystallographic co-ordinates for DAF(34) and EV11 into the reconstruction shows a close agreement between the crystal structure of the receptor fragment and the density for the virus-bound receptor, allowing unambiguous positioning of the receptor with respect to the virion (PDB code 1UPN). Our finding that the mode of virus-receptor interaction in EV12 is distinct from that seen for EV7 raises interesting questions regarding the evolution and biological significance of the DAF binding phenotype in these viruses.
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==About this Structure==
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The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55).,Bhella D, Goodfellow IG, Roversi P, Pettigrew D, Chaudhry Y, Evans DJ, Lea SM J Biol Chem. 2004 Feb 27;279(9):8325-32. Epub 2003 Nov 21. PMID:14634014<ref>PMID:14634014</ref>
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1UPN is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] and [[http://en.wikipedia.org/wiki/Human_echovirus_11 Human echovirus 11]]. Structure known Active Site: RB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1UPN OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The structure of echovirus type 12 bound to a two-domain fragment of its cellular attachment protein decay-accelerating factor (CD 55)., Bhella D, Goodfellow IG, Roversi P, Pettigrew D, Chaudhry Y, Evans DJ, Lea SM, J Biol Chem. 2004 Feb 27;279(9):8325-32. Epub 2003 Nov 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14634014 14634014]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1upn" style="background-color:#fffaf0;"></div>
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[[Category: Human echovirus 11]]
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[[Category: Protein complex]]
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[[Category: Bhella, D.]]
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[[Category: Chaudry, Y.]]
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[[Category: Evans, D.J.]]
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[[Category: Goodfellow, I.G.]]
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[[Category: Lea, S.M.]]
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[[Category: Pettigrew, D.]]
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[[Category: Roversi, P.]]
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[[Category: cd55]]
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[[Category: daf]]
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[[Category: echovirus]]
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[[Category: electron microscopy]]
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[[Category: icosahedral virus]]
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[[Category: picornavirus]]
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[[Category: virus-receptor complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:21:01 2007''
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==See Also==
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*[[CD55|CD55]]
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
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</SX>
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[[Category: Echovirus E11]]
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Bhella D]]
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[[Category: Chaudry Y]]
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[[Category: Evans DJ]]
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[[Category: Goodfellow IG]]
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[[Category: Lea SM]]
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[[Category: Pettigrew D]]
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[[Category: Roversi P]]

Current revision

COMPLEX OF ECHOVIRUS TYPE 12 WITH DOMAINS 3 AND 4 OF ITS RECEPTOR DECAY ACCELERATING FACTOR (CD55) BY CRYO ELECTRON MICROSCOPY AT 16 A

1upn, resolution 16.00Å

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