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1uv7

From Proteopedia

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Current revision

periplasmic domain of EpsM from Vibrio cholerae

Structural highlights

1uv7 is a 2 chain structure with sequence from Vibrio cholerae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GSPM_VIBCH Inner membrane component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Plays a role in the complex assembly and recruits EpsL resulting in a stable complex in the inner membrane. Provides thus a link between the energy-providing EpsE protein in the cytoplasm and the rest of the T2SS machinery.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The terminal branch of the general secretion pathway (Gsp or type II secretion system) is used by several pathogenic bacteria for the secretion of their virulence factors across the outer membrane. In these secretion systems, a complex of 12-15 Gsp proteins spans from the pore in the outer membrane via several associated signal or energy-transducing proteins in the inner membrane to a regulating ATPase in the cytosol. The human pathogen Vibrio cholerae uses such a system, called the Eps system, for the export of the cholera toxin and other virulence factors from its periplasm into the lumen of the gastrointestinal tract of the host. Here, we report the atomic structure of the periplasmic domain of the EpsM protein from V.cholerae, which is a part of the interface between the regulating part and the rest of the Eps system. The crystal structure was determined by Se-Met MAD phasing and the model was refined to 1.7A resolution. The monomer consists of two alphabetabeta-subdomains forming a sandwich of two alpha-helices and a four-stranded antiparallel beta-sheet. In the dimer, a deep cleft with a polar rim and a hydrophobic bottom made by conserved residues is located between the monomers. This cleft contains an extra electron density suggesting that this region might serve as a binding site of an unknown ligand or part of a protein partner. Unexpectedly, the fold of the periplasmic domain of EpsM is an undescribed circular permutation of the ferredoxin fold.

The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin fold.,Abendroth J, Rice AE, McLuskey K, Bagdasarian M, Hol WG J Mol Biol. 2004 Apr 30;338(3):585-96. PMID:15081815^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sandkvist M, Hough LP, Bagdasarian MM, Bagdasarian M. Direct interaction of the EpsL and EpsM proteins of the general secretion apparatus in Vibrio cholerae. J Bacteriol. 1999 May;181(10):3129-35. PMID:10322014 doi:10.1128/JB.181.10.3129-3135.1999
↑ Sandkvist M, Keith JM, Bagdasarian M, Howard SP. Two regions of EpsL involved in species-specific protein-protein interactions with EpsE and EpsM of the general secretion pathway in Vibrio cholerae. J Bacteriol. 2000 Feb;182(3):742-8. PMID:10633109 doi:10.1128/JB.182.3.742-748.2000
↑ Lybarger SR, Johnson TL, Gray MD, Sikora AE, Sandkvist M. Docking and assembly of the type II secretion complex of Vibrio cholerae. J Bacteriol. 2009 May;191(9):3149-61. PMID:19251862 doi:10.1128/JB.01701-08
↑ Abendroth J, Rice AE, McLuskey K, Bagdasarian M, Hol WG. The crystal structure of the periplasmic domain of the type II secretion system protein EpsM from Vibrio cholerae: the simplest version of the ferredoxin fold. J Mol Biol. 2004 Apr 30;338(3):585-96. PMID:15081815 doi:10.1016/j.jmb.2004.01.064

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1uv7"

Categories: Large Structures | Vibrio cholerae | Abendroth J | Hol WGJ

@@ Line 1: / Line 1: @@
-==PERIPLASMIC DOMAIN OF EPSM FROM VIBRIO CHOLERAE==
-<StructureSection load='1uv7' size='340' side='right' caption='[[1uv7]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+==periplasmic domain of EpsM from Vibrio cholerae==
+<StructureSection load='1uv7' size='340' side='right'caption='[[1uv7]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1uv7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UV7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UV7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1uv7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UV7 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uv7 OCA], [http://pdbe.org/1uv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uv7 RCSB], [http://www.ebi.ac.uk/pdbsum/1uv7 PDBsum]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uv7 OCA], [https://pdbe.org/1uv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uv7 RCSB], [https://www.ebi.ac.uk/pdbsum/1uv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uv7 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/GSPM_VIBCH GSPM_VIBCH] Inner membrane component of the type II secretion system required for the energy-dependent secretion of extracellular factors such as proteases and toxins from the periplasm. Plays a role in the complex assembly and recruits EpsL resulting in a stable complex in the inner membrane. Provides thus a link between the energy-providing EpsE protein in the cytoplasm and the rest of the T2SS machinery.<ref>PMID:10322014</ref> <ref>PMID:10633109</ref> <ref>PMID:19251862</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/1uv7_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uv/1uv7_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 25: / Line 29: @@
 </div>
 <div class="pdbe-citations 1uv7" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[General secretion pathway protein 3D structures|General secretion pathway protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bacillo virgola del koch trevisan 1884]]
+[[Category: Large Structures]]
-[[Category: Abendroth, J]]
-[[Category: Hol, W G.J]]
-[[Category: General secretion pathway]]
-[[Category: Transport]]
 [[Category: Vibrio cholerae]]
+[[Category: Abendroth J]]
+[[Category: Hol WGJ]]

1uv7

From Proteopedia

Current revision

periplasmic domain of EpsM from Vibrio cholerae

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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