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1uw0

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[[Image:1uw0.gif|left|200px]]
 
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{{Structure
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==Solution structure of the zinc-finger domain from DNA ligase IIIa==
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|PDB= 1uw0 |SIZE=350|CAPTION= <scene name='initialview01'>1uw0</scene>
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<StructureSection load='1uw0' size='340' side='right'caption='[[1uw0]]' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1uw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UW0 FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_ligase_(ATP) DNA ligase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.5.1.1 6.5.1.1] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uw0 OCA], [https://pdbe.org/1uw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uw0 RCSB], [https://www.ebi.ac.uk/pdbsum/1uw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uw0 ProSAT]</span></td></tr>
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|RELATEDENTRY=
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uw0 OCA], [http://www.ebi.ac.uk/pdbsum/1uw0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uw0 RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/DNLI3_HUMAN DNLI3_HUMAN] Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uw/1uw0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uw0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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DNA ligase IIIalpha carries out the final ligation step in the base excision repair (BER) and single strand break repair (SSBR) mechanisms of DNA repair. The enzyme recognises single-strand nicks and other damage features in double-stranded DNA, both through the catalytic domain and an N-terminal domain containing a single zinc finger. The latter is homologous to other zinc fingers that recognise damaged DNA, two in the N terminus of poly(adenosine-ribose)polymerase and three in the N terminus of the Arabidopsis thaliana nick-sensing DNA 3'-phosphoesterase. Here, we present the solution structure of the zinc-finger domain of human DNA ligase IIIalpha, the first structure of a finger from this group. It is related to that of the erythroid transcription factor GATA-1, but has an additional N-terminal beta-strand and C-terminal alpha-helix. Chemical shift mapping using a DNA ligand containing a single-stranded break showed that the DNA-binding surface of the DNA-ligase IIIalpha zinc finger is substantially different from that of GATA-1, consistent with the fact that the two proteins recognise very different features in the DNA. Likely implications for DNA binding are discussed.
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'''SOLUTION STRUCTURE OF THE ZINC-FINGER DOMAIN FROM DNA LIGASE IIIA'''
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Solution structure and DNA binding of the zinc-finger domain from DNA ligase IIIalpha.,Kulczyk AW, Yang JC, Neuhaus D J Mol Biol. 2004 Aug 13;341(3):723-38. PMID:15288782<ref>PMID:15288782</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1uw0" style="background-color:#fffaf0;"></div>
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==Overview==
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==See Also==
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DNA ligase IIIalpha carries out the final ligation step in the base excision repair (BER) and single strand break repair (SSBR) mechanisms of DNA repair. The enzyme recognises single-strand nicks and other damage features in double-stranded DNA, both through the catalytic domain and an N-terminal domain containing a single zinc finger. The latter is homologous to other zinc fingers that recognise damaged DNA, two in the N terminus of poly(adenosine-ribose)polymerase and three in the N terminus of the Arabidopsis thaliana nick-sensing DNA 3'-phosphoesterase. Here, we present the solution structure of the zinc-finger domain of human DNA ligase IIIalpha, the first structure of a finger from this group. It is related to that of the erythroid transcription factor GATA-1, but has an additional N-terminal beta-strand and C-terminal alpha-helix. Chemical shift mapping using a DNA ligand containing a single-stranded break showed that the DNA-binding surface of the DNA-ligase IIIalpha zinc finger is substantially different from that of GATA-1, consistent with the fact that the two proteins recognise very different features in the DNA. Likely implications for DNA binding are discussed.
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*[[DNA ligase 3D structures|DNA ligase 3D structures]]
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== References ==
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==About this Structure==
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<references/>
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1UW0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UW0 OCA].
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__TOC__
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</StructureSection>
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==Reference==
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Solution structure and DNA binding of the zinc-finger domain from DNA ligase IIIalpha., Kulczyk AW, Yang JC, Neuhaus D, J Mol Biol. 2004 Aug 13;341(3):723-38. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15288782 15288782]
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[[Category: DNA ligase (ATP)]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kulczyk, A W.]]
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[[Category: Kulczyk AW]]
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[[Category: Neuhaus, D.]]
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[[Category: Neuhaus D]]
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[[Category: Yang, J C.]]
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[[Category: Yang J-C]]
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[[Category: cell division]]
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[[Category: dna repair]]
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[[Category: dna replication]]
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[[Category: ligase]]
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[[Category: nuclear protein]]
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[[Category: parp-like finger]]
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[[Category: zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:16:01 2008''
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Current revision

Solution structure of the zinc-finger domain from DNA ligase IIIa

PDB ID 1uw0

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