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1w6s
From Proteopedia
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(New page: 200px<br /> <applet load="1w6s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1w6s, resolution 1.2Å" /> '''THE HIGH RESOLUTION ...) |
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| - | [[Image:1w6s.gif|left|200px]]<br /> | ||
| - | <applet load="1w6s" size="450" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="1w6s, resolution 1.2Å" /> | ||
| - | '''THE HIGH RESOLUTION STRUCTURE OF METHANOL DEHYDROGENASE FROM METHYLOBACTERIUM EXTORQUENS'''<br /> | ||
| - | == | + | ==The high resolution structure of methanol dehydrogenase from methylobacterium extorquens== |
| - | The crystal structure of methanol dehydrogenase (MDH) from | + | <StructureSection load='1w6s' size='340' side='right'caption='[[1w6s]], [[Resolution|resolution]] 1.20Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1w6s]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W6S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W6S FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w6s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w6s OCA], [https://pdbe.org/1w6s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w6s RCSB], [https://www.ebi.ac.uk/pdbsum/1w6s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w6s ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/DHM1_METEA DHM1_METEA] Catalyzes the oxidation of primary alcohols including methanol. | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w6/1w6s_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w6s ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The crystal structure of methanol dehydrogenase (MDH) from Methylobacterium extorquens has been refined without stereochemical restraints at a resolution of 1.2 A. The high-resolution data have defined the conformation of the tricyclic pyrroloquinoline quinone (PQQ) cofactor ring as entirely planar. The detailed definition of the active-site geometry has shown many features that are similar to the quinohaemo-protein alcohol dehydrogenases from Comamonas testosteroni and Pseudomonas putida, both of which possess MDH-like and cytochrome c-like domains. Conserved features between the two types of PQQ-containing enzyme suggest a common pathway for electron transfer between MDH and its physiological electron acceptor cytochrome cL. A pathway for proton transfer from the active site to the bulk solvent is also suggested. | ||
| - | + | The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens.,Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB Acta Crystallogr D Biol Crystallogr. 2005 Jan;61(Pt 1):75-9. Epub 2004 Dec, 17. PMID:15608378<ref>PMID:15608378</ref> | |
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| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 1w6s" style="background-color:#fffaf0;"></div> | |
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| - | + | ==See Also== | |
| + | *[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]] | ||
| + | *[[Methanol dehydrogenase|Methanol dehydrogenase]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Methylorubrum extorquens]] | ||
| + | [[Category: Anthony C]] | ||
| + | [[Category: Coates L]] | ||
| + | [[Category: Cooper JB]] | ||
| + | [[Category: Erskine PT]] | ||
| + | [[Category: Gill R]] | ||
| + | [[Category: Mohammed F]] | ||
| + | [[Category: Williams PA]] | ||
| + | [[Category: Wood SP]] | ||
Current revision
The high resolution structure of methanol dehydrogenase from methylobacterium extorquens
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