1wp9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1wp9" [edit=sysop:move=sysop])
Current revision (13:46, 9 May 2024) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1wp9.png|left|200px]]
 
-
{{STRUCTURE_1wp9| PDB=1wp9 | SCENE= }}
+
==Crystal structure of Pyrococcus furiosus Hef helicase domain==
 +
<StructureSection load='1wp9' size='340' side='right'caption='[[1wp9]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1wp9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WP9 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wp9 OCA], [https://pdbe.org/1wp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wp9 RCSB], [https://www.ebi.ac.uk/pdbsum/1wp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wp9 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q8TZH8_PYRFU Q8TZH8_PYRFU]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wp/1wp9_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wp9 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
DNA and RNA frequently form various branched intermediates that are important for the transmission of genetic information. Helicases play pivotal roles in the processing of these transient intermediates during nucleic acid metabolism. The archaeal Hef helicase/ nuclease is a representative protein that processes flap- or fork-DNA structures, and, intriguingly, its C-terminal half belongs to the XPF/Mus81 nuclease family. Here, we report the crystal structure of the helicase domain of the Hef protein from Pyrococcus furiosus. The structure reveals a novel helical insertion between the two conserved helicase core domains. This positively charged extra region, structurally similar to the "thumb" domain of DNA polymerase, plays critical roles in fork recognition. The Hef helicase/nuclease exhibits sequence similarity to the Mph1 helicase from Saccharomyces cerevisiae; XPF/Rad1, involved in DNA repair; and a putative Hef homolog identified in mammals. Hence, our findings provide a structural basis for the functional mechanisms of this helicase/nuclease family.
-
===Crystal structure of Pyrococcus furiosus Hef helicase domain===
+
Crystal structure and functional implications of Pyrococcus furiosus hef helicase domain involved in branched DNA processing.,Nishino T, Komori K, Tsuchiya D, Ishino Y, Morikawa K Structure. 2005 Jan;13(1):143-53. PMID:15642269<ref>PMID:15642269</ref>
-
{{ABSTRACT_PUBMED_15642269}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1wp9" style="background-color:#fffaf0;"></div>
-
[[1wp9]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus_dsm_3638 Pyrococcus furiosus dsm 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WP9 OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:015642269</ref><references group="xtra"/>
+
</StructureSection>
-
[[Category: Adenosinetriphosphatase]]
+
[[Category: Large Structures]]
-
[[Category: Pyrococcus furiosus dsm 3638]]
+
[[Category: Pyrococcus furiosus DSM 3638]]
-
[[Category: Ishino, Y.]]
+
[[Category: Ishino Y]]
-
[[Category: Komori, K.]]
+
[[Category: Komori K]]
-
[[Category: Morikawa, K.]]
+
[[Category: Morikawa K]]
-
[[Category: Nishino, T.]]
+
[[Category: Nishino T]]
-
[[Category: Tsuchiya, D.]]
+
[[Category: Tsuchiya D]]
-
[[Category: Atpase]]
+
-
[[Category: Dna recombination]]
+
-
[[Category: Dna repair]]
+
-
[[Category: Dna replication]]
+
-
[[Category: Helicase]]
+
-
[[Category: Hydrolase]]
+

Current revision

Crystal structure of Pyrococcus furiosus Hef helicase domain

PDB ID 1wp9

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wp9"
Personal tools