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1wzm

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[[Image:1wzm.gif|left|200px]]
 
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{{Structure
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==Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt R469K==
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|PDB= 1wzm |SIZE=350|CAPTION= <scene name='initialview01'>1wzm</scene>, resolution 3.20&Aring;
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<StructureSection load='1wzm' size='340' side='right'caption='[[1wzm]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
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<table><tr><td colspan='2'>[[1wzm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WZM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WZM FirstGlance]. <br>
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Neopullulanase Neopullulanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.135 3.2.1.135] </span>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wzm OCA], [https://pdbe.org/1wzm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wzm RCSB], [https://www.ebi.ac.uk/pdbsum/1wzm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wzm ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1ji2|1JI2]], [[1wzk|1WZK]], [[1wzl|1WZL]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wzm OCA], [http://www.ebi.ac.uk/pdbsum/1wzm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wzm RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/NEPU2_THEVU NEPU2_THEVU] Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wz/1wzm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wzm ConSurf].
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<div style="clear:both"></div>
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'''Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt R469K'''
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==See Also==
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*[[Amylase 3D structures|Amylase 3D structures]]
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__TOC__
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==About this Structure==
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</StructureSection>
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1WZM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermoactinomyces_vulgaris Thermoactinomyces vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WZM OCA].
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[[Category: Large Structures]]
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[[Category: Neopullulanase]]
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[[Category: Single protein]]
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[[Category: Thermoactinomyces vulgaris]]
[[Category: Thermoactinomyces vulgaris]]
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[[Category: Ichikawa, K.]]
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[[Category: Ichikawa K]]
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[[Category: Kamitori, S.]]
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[[Category: Kamitori S]]
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[[Category: Mizuno, M.]]
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[[Category: Mizuno M]]
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[[Category: Nishikawa, A.]]
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[[Category: Nishikawa A]]
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[[Category: Ohtaki, A.]]
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[[Category: Ohtaki A]]
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[[Category: Sakano, Y.]]
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[[Category: Sakano Y]]
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[[Category: Shimura, Y.]]
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[[Category: Shimura Y]]
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[[Category: Tonozuka, T.]]
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[[Category: Tonozuka T]]
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[[Category: alpha-amylase]]
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[[Category: alpha-amylase family]]
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[[Category: gh-13]]
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[[Category: pullulan]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:43:01 2008''
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Current revision

Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) mutatnt R469K

PDB ID 1wzm

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