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1e3t

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(New page: 200px<br /><applet load="1e3t" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e3t" /> '''SOLUTION STRUCTURE OF THE NADP(H) BINDING CO...)
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[[Image:1e3t.jpg|left|200px]]<br /><applet load="1e3t" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1e3t" />
 
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'''SOLUTION STRUCTURE OF THE NADP(H) BINDING COMPONENT (DIII) OF PROTON-TRANSLOCATING TRANSHYDROGENASE FROM RHODOSPIRILLUM RUBRUM'''<br />
 
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==Overview==
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==Solution Structure of the NADP(H) binding Component (dIII) of Proton-Translocating Transhydrogenase from Rhodospirillum rubrum==
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Transhydrogenase is a proton pump found in the membranes of bacteria and, animal mitochondria. The solution structure of the expressed, 21.5 kDa, NADP(H)-binding component (dIII) of transhydrogenase from Rhodospirillum, rubrum has been solved by NMR methods. This is the first description of, the structure of dIII from a bacterial source. The protein adopts a, Rossmann fold: an open, twisted, parallel beta-sheet, flanked by helices., However, the binding of NADP(+) to dIII is profoundly different to that, seen in other Rossmann structures, in that its orientation is reversed:, the adenosine moiety interacts with the first betaalphabetaalphabeta, motif, and the nicotinamide with the second. Features in the structure, that might be responsible for changes in nucleotide-binding affinity, during catalysis, and for interaction with other components of the enzyme, are identified. The results are compared with the recently determined, high-resolution crystal structures of human and bovine dIII which also, show the reversed nucleotide orientation.
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<StructureSection load='1e3t' size='340' side='right'caption='[[1e3t]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1e3t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E3T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3t OCA], [https://pdbe.org/1e3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e3t RCSB], [https://www.ebi.ac.uk/pdbsum/1e3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e3t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PNTB_RHORT PNTB_RHORT] The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.[UniProtKB:P07001]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e3/1e3t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1e3t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Transhydrogenase is a proton pump found in the membranes of bacteria and animal mitochondria. The solution structure of the expressed, 21.5 kDa, NADP(H)-binding component (dIII) of transhydrogenase from Rhodospirillum rubrum has been solved by NMR methods. This is the first description of the structure of dIII from a bacterial source. The protein adopts a Rossmann fold: an open, twisted, parallel beta-sheet, flanked by helices. However, the binding of NADP(+) to dIII is profoundly different to that seen in other Rossmann structures, in that its orientation is reversed: the adenosine moiety interacts with the first betaalphabetaalphabeta motif, and the nicotinamide with the second. Features in the structure that might be responsible for changes in nucleotide-binding affinity during catalysis, and for interaction with other components of the enzyme, are identified. The results are compared with the recently determined, high-resolution crystal structures of human and bovine dIII which also show the reversed nucleotide orientation.
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==About this Structure==
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Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum.,Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB Biochim Biophys Acta. 2000 Aug 15;1459(2-3):248-57. PMID:11004437<ref>PMID:11004437</ref>
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1E3T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodospirillum_rubrum Rhodospirillum rubrum] with NAP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/NAD(P)(+)_transhydrogenase_(B-specific) NAD(P)(+) transhydrogenase (B-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.1.1 1.6.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E3T OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from Rhodospirillum rubrum., Jeeves M, Smith KJ, Quirk PG, Cotton NP, Jackson JB, Biochim Biophys Acta. 2000 Aug 15;1459(2-3):248-57. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11004437 11004437]
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</div>
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[[Category: NAD(P)(+) transhydrogenase (B-specific)]]
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<div class="pdbe-citations 1e3t" style="background-color:#fffaf0;"></div>
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[[Category: Rhodospirillum rubrum]]
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[[Category: Single protein]]
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[[Category: Cotton, N.P.J.]]
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[[Category: Jackson, J.B.]]
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[[Category: Jeeves, M.]]
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[[Category: Quirk, P.G.]]
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[[Category: Smith, K.J.]]
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[[Category: NAP]]
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[[Category: membrane protein]]
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[[Category: nmr structure]]
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[[Category: nucleotide binding]]
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[[Category: proton translocation]]
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[[Category: transhydrogenase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:45:53 2007''
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==See Also==
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*[[NAD(P) transhydrogenase 3D structures|NAD(P) transhydrogenase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Rhodospirillum rubrum]]
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[[Category: Cotton NPJ]]
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[[Category: Jackson JB]]
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[[Category: Jeeves M]]
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[[Category: Quirk PG]]
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[[Category: Smith KJ]]

Current revision

Solution Structure of the NADP(H) binding Component (dIII) of Proton-Translocating Transhydrogenase from Rhodospirillum rubrum

PDB ID 1e3t

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