1h0t

From Proteopedia

(Difference between revisions)

Current revision

An affibody in complex with a target protein: structure and coupled folding

Structural highlights

1h0t is a 2 chain structure with sequence from Staphylococcus aureus and Synthetic construct. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SPA_STAA8

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Combinatorial protein engineering provides powerful means for functional selection of novel binding proteins. One class of engineered binding proteins, denoted affibodies, is based on the three-helix scaffold of the Z domain derived from staphylococcal protein A. The Z(SPA-1) affibody has been selected from a phage-displayed library as a binder to protein A. Z(SPA-1) also binds with micromolar affinity to its own ancestor, the Z domain. We have characterized the Z(SPA-1) affibody in its uncomplexed state and determined the solution structure of a Z:Z(SPA-1) protein-protein complex. Uncomplexed Z(SPA-1) behaves as an aggregation-prone molten globule, but folding occurs on binding, and the original (Z) three-helix bundle scaffold is fully formed in the complex. The structural basis for selection and strong binding is a large interaction interface with tight steric and polar/nonpolar complementarity that directly involves 10 of 13 mutated amino acid residues on Z(SPA-1). We also note similarities in how the surface of the Z domain responds by induced fit to binding of Z(SPA-1) and Ig Fc, respectively, suggesting that the Z(SPA-1) affibody is capable of mimicking the morphology of the natural binding partner for the Z domain.

An affibody in complex with a target protein: structure and coupled folding.,Wahlberg E, Lendel C, Helgstrand M, Allard P, Dincbas-Renqvist V, Hedqvist A, Berglund H, Nygren PA, Hard T Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3185-90. Epub 2003 Feb 19. PMID:12594333^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wahlberg E, Lendel C, Helgstrand M, Allard P, Dincbas-Renqvist V, Hedqvist A, Berglund H, Nygren PA, Hard T. An affibody in complex with a target protein: structure and coupled folding. Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3185-90. Epub 2003 Feb 19. PMID:12594333 doi:10.1073/pnas.0436086100

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1h0t"

@@ Line 1: / Line 1: @@
-==AN AFFIBODY IN COMPLEX WITH A TARGET PROTEIN: STRUCTURE AND COUPLED FOLDING==
-<StructureSection load='1h0t' size='340' side='right' caption='[[1h0t]], [[NMR_Ensembles_of_Models | 40 NMR models]]' scene=''>
+==An affibody in complex with a target protein: structure and coupled folding==
+<StructureSection load='1h0t' size='340' side='right'caption='[[1h0t]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1h0t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H0T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1H0T FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1h0t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H0T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H0T FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1dee|1dee]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1h0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h0t OCA], [http://pdbe.org/1h0t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1h0t RCSB], [http://www.ebi.ac.uk/pdbsum/1h0t PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h0t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h0t OCA], [https://pdbe.org/1h0t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h0t RCSB], [https://www.ebi.ac.uk/pdbsum/1h0t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h0t ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/SPA_STAA8 SPA_STAA8]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h0/1h0t_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h0/1h0t_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Allard, P]]
+[[Category: Large Structures]]
-[[Category: Berglund, H]]
+[[Category: Staphylococcus aureus]]
-[[Category: Dincbas-Renqvist, V]]
+[[Category: Synthetic construct]]
-[[Category: Hard, T]]
+[[Category: Allard P]]
-[[Category: Hedqvist, A]]
+[[Category: Berglund H]]
-[[Category: Helgstrand, M]]
+[[Category: Dincbas-Renqvist V]]
-[[Category: Lendel, C]]
+[[Category: Hard T]]
-[[Category: Nygren, P A]]
+[[Category: Hedqvist A]]
-[[Category: Wahlberg, E]]
+[[Category: Helgstrand M]]
-[[Category: Affibody]]
+[[Category: Lendel C]]
-[[Category: Coupled protein folding]]
+[[Category: Nygren P-A]]
-[[Category: Igg binding protein some]]
+[[Category: Wahlberg E]]
-[[Category: Immune system]]
-[[Category: Induced fit]]
-[[Category: Molecular recognition]]
-[[Category: Molten globule]]
-[[Category: Nmr spectroscopy]]
-[[Category: Protein engineering]]
-[[Category: Protein-protein interaction]]

1h0t

From Proteopedia

Current revision

An affibody in complex with a target protein: structure and coupled folding

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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